摘要
目的探讨前列腺特异抗原(PSA)mRNA和血管内皮细胞生长因子(VEGF)mRNA在乳腺癌组织中的表达及其关系。方法采用半定量逆转录聚合酶链式反应(RT-PCR)检测35例乳腺癌、12例癌旁乳腺组织和10例乳腺纤维腺瘤组织中的表达,免疫组化检测35例乳腺癌组织中ER、PR的表达,分析PSA mRNA和VEGFmRNA表达之间的关系。结果PSA mRNA在乳腺癌组织中的表达水平明显低于癌旁乳腺组织和乳腺纤维腺瘤组织,差异有显著性(P值均=0.00)。VEGF 121、165mRNA在乳腺癌中的表达水平明显高于癌旁乳腺组织和乳腺纤维腺瘤组织的表达(P值均=0.00)。ER、PR阳性表达的乳腺癌组织中PSA mRNA表达高于ER、PR阴性者,差异有显著性(P=0.001和0.004)。PSA mRNA表达阳性者VEGF 121、165mRNA表达水平明显低于PSA mRNA表达阴性者(P=0.034、0.026)。结论PSA mRNA在乳腺癌组织中的表达下调,但其表达受ER、PR的调控,而且其可能具有抑制乳腺肿瘤血管生成的作用。
Objective To investigate the expression of prostate-specific antigen mRNA （PSA mRNA）and vascular endothelial growth factor mRNA （VEGF mRNA） in breast cancer. Methods Semi-quantitive reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA and VEGF mRNA in 35 specimens of breast cancer, 12 of paracarcinoma breast tissues and 10 of breast adenofibroma. Immunohistochemistry method was conducted to analyze the expression of ER and PR protein. The correlation between the expression of PSA mRNA and VEGF mRNA was analyzed. Results The level of PSA mRNA in breast cancer was significantly lower than that in paracarcinoma breast tissues and breast adenofibroma tissues （both P= 0. 000）. The expression of VEGF121,165mRNA in breast cancer tissues was significantly higher than that in paracarcinoma breast tissues and breast adenofibroma tissues（all P=0. 000）. The expression of PSA mRNA in ER and PR positive breast cancer was higher than that in ER and PR negative （P= 0. 001,0. 004, respectively）. The expression of VEGF121,165mRNA in breast cancer with lymph node metastasis was significantly higher than that without lymph node metastasis（P=0. 033,0. 004）. The expression of VEGF121,165mRNA in PSA mRNA positive expression breast cancer tissues was significantly lower than that in PSA mRNA negative breast cancer（P = 0. 034 and 0. 026, respectively）. Conclusion PSA mRNA in breast cancer is downregulated, and its expression is related with ER and PR statue,and the expression of PSA mRNA might inhibit the angiogenesis of breast cancer.
出处
《实用肿瘤杂志》
CAS
2007年第4期300-303,共4页
Journal of Practical Oncology
