摘要
目的检测丙型肝炎患者血清肝特异性自身抗体并分析其临床意义。方法采用间接免疫荧光法,对220例丙型肝炎病毒感染者血清进行肝特异性自身抗体的检测;应用聚合酶链反应定量检测血清HCV-RNA含量,HCV抗体的检测用ELISA法;结果220例丙型肝炎患者总自身抗体检出率为34.55%;单纯Ant-HCV阳性的检出率为29.57%;HCV RNA和Ant-HCV均阳性自身抗体检出率为40.59%,单纯Ant-HCV阳性与HCV-RNA和Ant-HCV均阳性组比较差异显著(P<0.01)。各组与正常对照比较差异均非常显著(P<0.01);自身抗体以低滴度为主,主要为抗核抗体。自身抗体阳性的检出与性别无关(P>0.05)而与年龄关系密切(P<0.01),且自身抗体随年龄的增加检出率升高。结论HCV感染可诱导自身免疫反应,使患者血清中出现多种自身抗体,检测其自身抗体及滴度对丙型肝炎患者的诊断和治疗有一定的参考价值。
Objective To study the clinical significance of determining serum liver specific autoantibodies in HCV patients. Methods The serum liver specific autoantibodies were detected by indirect immunofluorescence assay,HCV-DNA was determined by real-time quantitative PCR and ant-HCV was measured with ELISA,from 220 patients with hepatitis C were analyzed in the study. Results The positive rate of autoantibodies in 220 patients with hepatitis C were 34.55%. The positive rate of autoantibodies in simple Ant-HCV（＋）,HCV-RNA（＋） sire Ant-HCV（＋） specimens were 29.27% and 40. 59% respectively. There was significant difference compared with the normal groups（P〈0.01）. They are low titer of autoantibodies was presented in hepatitis C patients,and most of them were ANA. Autoantibodies were significantly associated with age （P〈0.01） ,hut not with gender （P〉0.05）. Conclusion HCV infection can induce autoimmune reactions resulting in production of autoantibodies and its titer is important indicates helpful for the diagnosis and treatment of HCV patients.
出处
《现代检验医学杂志》
CAS
2007年第4期28-30,共3页
Journal of Modern Laboratory Medicine
