摘要
背景:聚甲基丙烯酸β羟乙酯水凝胶在生物体多器官的应用显示了良好的生物相容性。目的:观察通过甲基丙烯酸改性聚甲基丙烯酸β羟乙酯水凝胶义眼台的生物相容性和纤维血管化情况。设计:单一样本观察。单位:解放军总医院眼科,清华大学化工系高分子研究所。材料:选择清洁级新西兰白兔25只,雌雄不拘,体质量2.0～2.5kg,由解放军总医院医学动物实验中心提供。按术后2,4,8,12,24周5个时间点进行观察,每个时间点5只。甲基丙烯酸改性聚甲基丙烯酸β羟乙酯水凝胶义眼台由清华大学化工系高分子研究所提供。实验经伦理学委员会批准。方法:实验于2003-03/10在解放军总医院眼科实验室完成。右眼为手术眼,麻醉后开睑器开睑,沿角膜缘全周剪开球结膜后分离筋膜囊,分离眼外肌并做预置缝线后剪断肌肉,剪断视神经后完整摘除眼球,将直径为14mm的甲基丙烯酸改性甲基丙烯酸β羟乙酯水凝胶义眼台植入兔眼眶内,术后2,4,8,12,24周做SPECT检查,并在术后2,4,8,12,24周取出植入物进行光镜、免疫组织化学及电镜检查。主要观察指标:在体情况下通过同位素示踪观察不同时间义眼台血管化状态。离体情况下通过光镜、免疫组织化学、电镜观察不同时间义眼台血管化状态。结果:纳入新西兰白兔25只全部进入结果分析。①SPECT检查显示2周时植入物周边有同位素浓集,4周和8周时范围逐渐向内扩张,在12周时植入物从周边到中央均有同位素浓集且分布均匀。②光镜结果显示MMA改性聚甲基丙烯酸β羟乙酯水凝胶义眼台纤维血管增长从周边到中心,2周时已有纤维血管组织长入植入物的孔隙,孔隙中可见到长或圆的细胞,核深染,为纤维母细胞。植入物孔隙内血管结构清晰,可见血管壁及血细胞。炎症细胞主要是中性粒细胞和淋巴细胞,偶尔可见大的巨噬细胞存在。③扫描电镜显示植入�
BACKGROUND： The application of polymerized 2-hydroxyethyl methacryate （PHEMA） in many organs of living body shows good biocompatibility.
OBJECTIVE： To observe the biocompatibility and fibrovascular condition of methl-methacrylate （MMA） modified PHEMA hydrogel orbital implant.
DESIGN： Single sample observation.
SETTING： Department of Ophthalmology, the General Hospital of Chinese PLA; Institute of Polymer Science,Department of Chemical Engineering, Tinghua university.
MATERIALS： Twenty-five New Zealand rabbits of either gender and clean grade, weighing 2.0 to 2.5 kg, were provided by the Animal Experimental Center, General Hospital of Chinese PLA. The animals were observed at postoperative 2, 4,8, 12 and 24 weeks, 5 at each time point. The experiment was permitted by ethics committee. MMA modified PHEMA hydrogel orbital implant was provided by Institute of Polymer Research, Department of Chemical Engineering, Tinghua University. The experiment was permitted by ethics committee.
METHODS： This experiment was carried out in the laboratory of Department of Ophthalmology, the General Hospital of Chinese PLA from March to October 2003. The right eye served as operation eye. When the rabbits were anesthetized,their eyelids were open with eyelid retractor. Bulbar conjunctiva was open along the edge of limbus of cornea for isolating fascia. Oculorotatory muscle was isolated and pre-set suture line was made, then muscle was cut off. When optical nerve was cut off, eyeball was completely excised. A MMA modified PHEMA hydrogel orbital implant with the diameter of 14 mm was implanted into the fossa orbitalis of rabbits. Single photon emission computed tomography （SPECT） was performed at postoperative 2, 4, 8, 12 and 24 weeks. The hydrogel orbital implant was taken out at postoperative 2, 4, 8,12 and 24 weeks for light microscope, immunohistochemical and electron microscope examinations.
MAIN OUTCOME MEASURES： Vascularization of hydrogel orbital implant was observed in vivo by isotope tracing obs
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第31期6290-6294,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
解放军总医院科研基金(06MP32)
军队医药卫生科研基金(06MA246)~~
