摘要
应用HPLC方法检测血清中索他洛尔(So-talol)浓度.以阿替洛尔(Atenolol)为内标,采用C18分析柱及保护柱,流动相为甲醇:乙腈:PB(10mmol/L pH4.0)(3:3:94V/V).流速为1.0ml/min.提取溶剂为三氯甲烷:异丙醇(4:1V/V),采用荧光检测器,激光波长及发射波长分别为235nm及313nm.实验结果表明:索他洛尔与内标分离良好,保留时间分别为5min与6.7min,在血清中萃取的绝对回收率分别为80.0%及81.5%,索他洛尔在0～5μg/ml范围内线性关系良好,相关系数r为0.9999,回归方程Y=0.000 5+1.7387X.日内及日间变异均小于6%.最低检测浓度0.01μg/ml.本方法适用于索他洛尔的药物动力学研究.
The analysis of sotalol in human serum by a simple and sensitive liquid chro-matographic assay is described. Analyses are carried out on a reversed-phase chro-matographic system using as octadecylsilane stationary phase and a methanol-acetonitrile-phosphate buffer (3: 3: 94 vol/vol/vol pH 4. 0). Atenolol is used as the internal standard (I. S. ). Sample preparation involves an extraction by chloroform and isopropyl alcohol (4 : 1 vol/vol/vol). The retention times of sotalol and I. S. are 5. 0 min and 6. 7 min respectively. Calibration is linear from 0μg/ml to 5.0μg/ml (r = 0. 999 9). The limit of detection is 0. 01μ/g/ml with a fluorometric detector at 235 nm EXλ and 313 nm EMλ. Within-day and day to day coefficients of variation are less than 6%. This assay appears to be suitable for pharmacokinetic studies as well as for therapeutic drug monitoring.
出处
《中国临床药学杂志》
CAS
1997年第1期22-24,共3页
Chinese Journal of Clinical Pharmacy
