摘要
目的:观察前列腺癌PC-3细胞株蛙皮素(Bombesin,BBS)受体蛋白和受体mRNA的表达。方法:采用免疫组织化学方法检测PC-3细胞中蛙皮素受体蛋白的表达,逆转录聚合酶链反应(RT-PCR)观察前列腺癌PC-3细胞株蛙皮素受体(BBS-R)mRNA的表达。结果:免疫组化方法检测PC-3细胞中有BBS-R蛋白的表达;RT-PCR产物与预期的BBS-R的cDNA分子量完全相符。结论:实验证明PC-3细胞存在有蛙皮素特异性受体,为探索蛙皮素和蛙皮素受体的拮抗剂应用于肿瘤研究以及蛙皮素作用后细胞内信息传递途径提供了基础。
Objective : To observe the expression of bombesin receptor mRNA and its protein in PC - 3 prostate cancer cells Line. Methods: Bombesin receptor protein expressions in PC-3 prostate cancer cells were detected by immunohistochemical method staining; RT - PCR was performed for detection of PC -3 cell peptide BBS - R mRNA in PC -3 prostate cancer cells. Results: The positive expression rate of bombesin receptor protein was significantly higher in PC -3 Cells;The molecular weight of the cDNA observed by RT - PCR was consistent with that of expected. Conclusion: By using immunohistochemical method and RT- PCR ,it was demonstrated that PC -3 prostate cancer cells can express bombesin receptor mRNA and protein. The results of this study provides a basis for further researches on bombesin intracellar signal transmission pathway and the role of bombesin and bombesin receptor antagonist in on tumour research.
出处
《现代肿瘤医学》
CAS
2007年第8期1069-1070,共2页
Journal of Modern Oncology
关键词
蛙皮素
聚合酶链反应
免疫组织化学
前列腺癌
bombesin
polymerase chain reaction
immunohistochemistry
prostate cancer