摘要
目的:建立以反相高效液相色谱法测定肾移植患者全血中环孢素A(CsA)浓度的方法。方法:采用单步萃取法提取,以反相高效液相色谱法测定,其中色谱柱为C8,流动相为乙腈-甲醇-水(45∶30∶25),流速为1.2mL·min-1,检测波长为210nm,柱温为60℃。结果:全血中CsA的检测浓度在50～600ng.mL-1范围内线性关系良好(r=0.9997);方法平均回收率为99.44%,日内、日间RSD均<4%;当S/N≥3时,方法的最低检测限为10ng.mL-1。结论:本方法准确、专一、回收率高、受代谢物干扰少、相对成本较低,可用于临床血药浓度检测及药动学研究。
OBJECTIVE： To establish a RP - HPLC method for the determination of the concentration of cyclosporin A （CsA） in whole blood of patients undergoing renal transplantation .METHODS： CsA was extracted from whole blood by single- step extraction and determined by RP - HPLC, and separated on a C8 column with a mobile phase consisted of acetonitrile- methanol- water（45 ： 30 ： 25） at a flow rate of 1.2mL·mL^-1.The detective wavelength was 210nm and the column temperature was maintained at 60℃ .RESULTS： The linear range of CsA was 50-600ng·mL^-1（ r = 0.999 7） .The average recovery rate of CsA was 99.44%, with both intra - day and inter - day RSD less than 4% .When S/N≥3, the detection limit of CsA was 10ng ·mL^-1.CONCLUSION：The established method was accurate and specific, meanwhile,it has high recovery,little interference and low cost, thus applicable for the determination of blood concentration and pharmacokinetics of CsA.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第23期1790-1791,共2页
China Pharmacy
