摘要
采用两种抗ＨＣＧ（人绒毛膜促性腺激素）单克隆抗体，固相试管包被技术建立了ＨＣＧ夹心免疫放射法（ＩＲＭＡ），与人促黄体激素、促卵泡激素和促甲状腺激素均无交叉反应。方法的灵敏度为０．５ＩＵ／Ｌ，批内、批间变异系数分别小于５．４％、６．４％。药盒４℃下存放１个月质量稳定。
An immunoradiometric assay (IRMA) for human chorionic gonadotropin (HCG) in serum is established based on two monoclonal antibodies against HCG. There are no cross-reaction with human luteinizing hormone (hLH), follicle stimulating hormone (hFSH) and thyroid stimulating hormone (hTSH). The sensitivity was 0.5U/L, and the recovery for different concentration of HCG was 91.65%￣107.62%. The coefficients of variation for intra and inter-assay were less than 5.4% and 6.4% respectively. 125 I-McAb were stable under the storage of 4℃ for 97 days.
出处
《核技术》
EI
CAS
CSCD
北大核心
1997年第5期313-316,共4页
Nuclear Techniques
