摘要
目的制备生物活性陶瓷细胞外基质复合材料,并通过动物体内骨植入研究,探讨人工骨材料对骨缺损的修复作用及相互作用机理,为骨替代材料临床应用提供依据。方法合成TCP、CHA、HA,并将其作为BMP的载体,制备成TCP/BMP、CHA/BMP复合材料,植入兔桡骨10mm骨缺损,同时加入TGF-β1,同时以单纯的TCP、CHA、HA材料组做对照。通过X线摄片及生物力学测定,扫描电镜及组织学染色分析等手段,观察新骨形成及材料的降解规律。结果术后动物均无不良反应。复合材料组植入2周时,骨断端部位可见软骨细胞;4周时可见新生软骨及骨组织,并向板层骨转化;8周时材料界面可见板层骨形成,TCP、CHA材料明显降解吸收,骨形成量明显增加;16周时材料界面新生骨趋向成熟,CHA材料降解区域由大量板层骨、骨髓组织填充;24周时新骨形成进一步增加,骨组织接近正常,但HA降解不明显,复合材料组新骨形成量明显高于相应的单纯材料。骨组织标本放射学及扫描电镜检测结果与组织观察结果基本一致。16周生物力学测定最大负载、弯曲及张力指标接近正常,与相应的单纯材料相比差异有显著性意义,说明细胞外基质的加入不仅有效地促进了新骨的形成,同时也加速了新骨的钙化。结论三种生物陶瓷是细胞外基质的理想载体,生物陶瓷基质复合材料对骨缺损有较强的修复能力,其中可降解生物陶瓷将是一类较为理想人工骨替代材料。
ObjectiveA newtype of composited artificial bone was constructed by combining bioceramic （TCP, CHA.HA） with extracell matrix （ECM） including bone morphogenetic protein （BMP） and transforming growth factor （TGF-β1 ）. it was same construction and mineralization as bone tissue,and osteoinductive of ECM. In orde to evaluate the feasibility to use his material to repair bone defect and related ostegenic potential in clinic, the abitily of biocerarmics were compared with TCP/BMP. CHA/BMP, HA/BMP composite in bone defects reconstruction by animal experiments. MethodsTyree bioceramics （TCP,CHA,HA） impregnated with ECM （experimental groups） and without ECM （control groups） were implant separately, bone defect about 10ram in the middle shaft of the radius of rabbits. The rabbits were sacyificed 2,4,8,16 and 24 weeks after operation. In order to observe the new bone formation and the degradation process of the materials, a series of examination were carried out including radiography inorganic subtance,biomechaically, scanning electron microscope （SEM） and histological detetion. Results The results of bioceramics group and composite material groups were compared on th same condition, and analysed the law of effect between bioactive ceramics and both of osteoblast and ECM. Detetion chondrocytes were found at 2nd week,and new lamellar bone and absorption of the composite materials were observed between 4weeks and 16 weeks,and tissue construction of new bone and mechanical strength reached almost to normal bone leel at 24 weeks. The speed of degradation of CHA/BMP was evidently higher than that other materials. The results of x - red . SEM examination were the same with histomorphological detection. Conclusion The biodegradable ceramics composite is one kind of good artifical bone substitute material to repair the bone defect.
出处
《北京生物医学工程》
2007年第4期401-407,424,共8页
Beijing Biomedical Engineering
基金
国家自然科学基金(30170273)资助
