摘要
目的:探讨人卵巢癌微血管内皮细胞与HUVEC在生物学特性及对血管生成因子刺激反应的差异。方法:分离纯化人卵巢癌微血管内皮细胞,以HUVEC为对照,用PDGF-BB刺激两种内皮细胞,利用免疫细胞化学和RT-PCR技术观察刺激后两种内皮细胞增殖、PCNA及VEGF表达的变化。结果:PDGF-BB(20ng/ml)能促进两种内皮细胞的增殖、PCNA和VEGF的表达,人卵巢癌微血管内皮细胞增殖和PCNA表达增强的幅度弱于HUVEC;而VEGF蛋白及mRNA表达增强的幅度高于HUVEC(P<0.05)。结论:人卵巢癌微血管内皮细胞与HUVEC对PDGF-BB刺激的反应有显著差异,为深入研究卵巢癌血管生成机制和抗血管生成治疗提供了实验模型。
Objective：To investigate the difference of biological characteristics and responses to PDGF-BB between human ovarian carcinoma-derived microvascular endothelial cells and human umbilical vein endothelial cells. Methods：The ovarian carcinoma-derived microvascular endothelial cells were isolated and purifed from ovarian carcinoma tissues. Compared to the human umbilical vein endothelial cells,the biological characteristics and responses to PDGF-BB were observed in OdMEC for the changes of growth,PCNA and VEGF expression. Results：20ng/ml PDGF-BB could enhance both kinds of endothelial cells to proliferate,express PCNA and VEGF. The proliferation and PCNA protein expression in HUVEC were augmented more significandy than those in OdMEC,the protein and mRNA of VEGF expression were augmented more significantly than those in HUVEC. Conclusion：The responses of OdMEC to PDGF-BB were significantly different from those of HUVEC,which provides experiment basis for research on ovarian carcinoma vascularization and antiangiogenesis therapy.
出处
《重庆医科大学学报》
CAS
CSCD
2007年第8期808-811,共4页
Journal of Chongqing Medical University
基金
第三军医大学校管青年科研基金资助项目(2002D076)
关键词
卵巢癌
血管内皮细胞
细胞培养
血小板源性生长因子
Ovarian carcinoma
Vascular endothelial cell
Cell culture
Platelet-derived growth factor
