摘要
目的探讨组织蛋白酶L(cathepsin L,CL)表达与大肠癌发生、发展及浸润转移的关系。方法应用原位杂交方法检测45例大肠癌组织及其相应的癌旁正常组织中CL mRNA的表达。结果正常大肠黏膜CL mRNA低表达,大肠癌组织CL mRNA阳性表达率明显高于正常组,两组间相比差异有统计学意义(P<0.05);Dukes'C和D级癌表达高于Dukes'A和B级(P<0.05);CL mRNA阳性表达率在中、低分化癌高于高分化癌。结论人大肠癌组织CL mRNA呈现高表达,CL与大肠癌的发展、浸润和转移有关。
Objective To explore the relations between expression of cathepsin L （CL） and development, invasion and metastasis of colorectal cancer . Methods The expression of CL mRNA in 45 cases with colorectal carcinoma and tumor-adjacent normal tissues are determined by in situ hybridization（ISH）. Results The expression of CL mRNA was low in normal eoloreetal mueosa, the positive rates of the expression of CL mRNA in the carcinoma tissues were higher obviously than those in normal colorectal mucosa . There was a significant difference between the two groups （ P 〈 0.01 ）. The positive rates of expression of CL mRNA in the carcinoma tissues of Dukes stage C and D were significantly higher than those of Dukes stage A and B （ P 〈 0.05 ）. The expression of CL mRNA was elevated in poorly differentiated cases than that of well differentiated. Conclusions The expression of CL mRNA in human colorectal cancer showed an obvious increase . CL is relative to development, invasion and metastasis of colorectal cancer.
出处
《肿瘤基础与临床》
2007年第1期9-11,共3页
journal of basic and clinical oncology
关键词
大肠癌
组织蛋白酶L
原位杂交
colorectal cancer
cathepsin L
in situ hybridization
