摘要
制备了水杨醛谷氨酸合镍修饰碳黑微电极，在JP-303极谱分析仪上研究了多巴胺（DA）在该修饰电极上的电化学行为，试验结果表明，在pH7．0的磷酸盐缓冲介质中，多巴胺在该修饰电极上其峰电流增强达3倍之多。产生一灵敏的氧化峰，在0．14V处峰电流与DA浓度在2．0×10^-7～1.0×10^-3mol·L^-1范围内呈线性关系，检出限（3σ）为1．0×10^-7mol·L^-1，应用于盐酸多巴胺注射剂中多巴胺的测定。测定结果的RSD（n=7）值小于3．5％，回收率为96％～103％。
Carbon black microelectrode modified by the coordination complex of nickel （Ⅱ） with N- salicylidene glutamic acid was prepared. Electrochemical behavior of dopamine at this modified microelectrode was studied by using the JP-303 polarograph. It was found that in a phosphate buffer of pH 7. 0, a sensitive oxidation peak was observed at 0. 14 V with 3 times enhanced peak current. Linear relationship was obtained between the peak current and the concentration of dopamine in the range of 2. 0× 10^-7 -1.0× 10^-3mol · L^-1 , with a detection limit （3s） of 1.0 × 10^-7 mol · L^-1. The proposed method was applied to the determination of dopamine in samples of dopamine hydrochloride injection, giving values of RSD＇s （n= 7） less than 3. 5%, and recoveries in the range of 96%-103%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2007年第7期534-536,539,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
伏安法
多巴胺
水杨醛谷氨酸合镍配合物
修饰碳黑微电极
Voltammetry
Dopamine
Coordination complex of Nickel（ Ⅱ ） with N-salicylidene-glutamic acid
Modified carbon black microelectrode
