摘要
目的探讨葛根素预处理对大鼠局灶性脑缺血再灌注时梗死范围和锥体细胞的影响。方法72只SD大鼠随机分为S组(假手术组)、IR组(缺血再灌注组)和P组(葛根素预处理组),每组24只,依术后处死动物时间的不同每组又分为3个亚组,每亚组8只,同时制作大脑中动脉缺血2h后再灌注损伤的SD大鼠模型。用TTC染色法测定梗死体积,HE染色观察海马区锥体细胞数。结果S组未见梗死灶,P组脑梗死范围明显比IR组缩小(P<0.05或P<0.01)。P24h、P72h组神经元损伤程度较IR24h、IR72h组轻;P24h、P72h组存活锥体细胞数明显多于IR24h、IR72h组(P=0.014,P=0.004)。结论葛根素预处理能减少大鼠局灶性脑缺血后梗死范围和锥体细胞的死亡。
Objective To investigate influence of puerarin preconditioning on infarction size and pyramidal cell after focal cerebral ischemia repeffusion injury in rats. Methods The rat model of middle cerebral artery occlusion （MCAO） were establishment. SD rats （n= 72） were randomly divided into three groups each consised of 24 rats. S group only exposed anatomically without reperfusion. Rats in IR group and P group were give reperfusion with administration of normal saline and puerarin through abdomen, respectively. Based on the hours after the mrs were killed, the rots in each group were divided into three subgroups each consisted of 8 rats. TIC staining was applied to determine infarction volume. The pathohistology in hippocampus was examined by HE staining. Results There no infarct foci were observed in S group. The infarct size of P group were significantly smaller than that of IR group, respectively （P 〈 0.05 or P 〈 0.01）. The extent of damange to hippocampus neurons in rats of P group were slighter significantly than that of IR group. The quantity of viable pyramidal cell in rats of P group at various time was significant mor than that of IR group （ P = 0.014, P = 0. 004）. Conclusion The preconditioning of puerarin can reduce the infarction area of focal cerebral ischemia due to reperfusion injury and protect the pyramdalcells in rats.
出处
《中国热带医学》
CAS
2007年第8期1303-1304,共2页
China Tropical Medicine
关键词
葛根素
预处理
梗死
缺血再灌注
锥体细胞
Puerarin
Preconditioning
Infarction
Ischemia repefusion
Pyramidal cell
