摘要
利用20个微卫星标记对3个虹鳟(Oncorhynchus mykiss)养殖群体进行遗传结构分析。结果表明,(1)3个群体检测的平均等位基因数为3·6～4·1,平均观测杂合度为0·5224～0·6328,平均期望杂合度为0·4736～0·5522,平均多态信息含量为0·4354～0·5084,说明这几个群体多态性属于偏高水平,遗传多样性高。通过d值,确定了Hard-Weinberg平衡的偏离情况,发现AY039638、AY039646在3个群体中都表现为不平衡。对3个群体的遗传距离进行了估算,并进行聚类分析,本溪的两个群体先聚为一支,再与渤海群体相聚,显示出明显的地理特征。(2)本溪虹鳟群体在位点AF352770出现部分等位基因消失的现象;AF352754在本溪群体中表现为位点消失,可作为区分本溪群体和渤海群体的分子标记。(3)综合评价3个群体,渤海站虹鳟群体的遗传多样性最高,与前人研究结果一致。
By using 20 microsatellite markers we determined polymorphism of three cultured Rainbow Trout （Oncorhynchus rnykiss ） populations. The average number of alleles was 3.85 ; the value of average observed heterozygosity （H） was 0.522 4 -0.632 8; the value of average expected heterozygosity （Ho） was 0.473 6 -0.552 2; and the value of average polymorphism information content （PIC） was 0.435 4 - 0.508 4. Hardyweinborg equilibrium （ d test） analysis revealed that the locus of AY039638 and AY039646 were lopsided in three populations, The genetic distance among the three cultured populations was calculated and the cluster analysis was also carried out. The phylogenetic tree showed that the two populations of Benxi had close relationship. In Benxi populations, the locus AF352754 and some alleles on AF352770 were lost. Benxi populations and Bohai population could be distinguished by locus AF352754. The Bohai population had the highest genetic diversity.
出处
《动物学杂志》
CAS
CSCD
北大核心
2007年第4期8-14,共7页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No2004CB117405)
关键词
虹鳟
微卫星
遗传多样性
Rainbow Trout （ Oncorhynchus mykiss ）
Microsatellite
Genetic diversity
