摘要
目的:探讨环氧化酶-2(cyclooxygenase-2,COX-2)与血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)在直肠腺癌中的表达和意义及其相关性。方法:免疫组织化学PV-8 000二步染色法,检测40例直肠腺癌原发灶标本和40例正常直肠黏膜中COX-2与VEGF-C蛋白的表达情况,并用血管内皮生长因子受-体3(vascular endothelial growth factor receptor,VEGFR-3)作为淋巴管内皮细胞的特异性标记物进行免疫组化染色,观察肿瘤组织淋巴管生成状况。结果:所测40例直肠腺癌组织中,COX-2及VEGF-C的表达阳性率分别为62.5%(25/40)和65.0%(26/40)。40例标本中的21例(52.5%)可以检测到VEGFR-3阳性的肿瘤新生淋巴管。COX-2表达与VEGF-C表达、肿瘤新生淋巴管形成及淋巴结转移、是否侵透浆膜层等临床病理指标间呈明显相关关系(P<0.05);但与肿瘤大小、肿瘤部位、病理组织学分型等无明显相关(P>0.05)。结论:直肠腺癌组织中COX-2表达与VEGF-C表达状态、肿瘤淋巴管生成和淋巴结转移均呈明显相关;COX-2可能通过上调VEGF-C表达促进直肠腺癌淋巴管生成,并顺次导致淋巴结转移的发生。
Objective： To investigate the expression of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor-C （VEGF-C） in human rectal adenocarcinoma. Methods： COX-2 and VEGF-C expressions were determined in 40 cases of rectal adenocarcinoma and 40 cases of normal rectal mucous membrane by the immunohistochemical PV-8000 two-step staining method. Lymphanigogenesis was evaluated by immunohistochemical staining with the specific antibody, VEGFR-3. Results： Expression rate of COX-2 and VEGF-C was 62.5% （25/40） and 65.0% （26/40）, respectively in 40 rectal adenocarcinoma specimens. VEGFR-3 was positive in 21 cases（21/40, 52.5%）indicating lymphangiogenesis in the tumors. Expression of COX-2 was significantly correlated with that of VEGF-C, tumor lymphangiogenesis and lymphatic metastasis and serosa invasion（P 〈 0.05）, however, it did not correlate with the tumor size, location and histological typing（ P 〈 0.05）. Conclusions： In rectal adenocarcinoma, expression of COX-2 is significantly associated with VEGF-C, lymphangiogenesis and lymphatic metastasis. COX-2 may up-regulate the expression of VEGF-C, which induces lymphangiogenesis and contributes to lymphatic metastasis.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第7期711-713,717,共4页
Journal of Shandong University:Health Sciences
关键词
直肠肿瘤
环氧化酶-2
淋巴管生成
淋巴转移
Rectal neoplasms
Cyclooxygenase-2
Lymphangiogenesis
Lymph node metastasis
