摘要
目的:确定化疗药丝裂霉素、长春新碱和5F-尿嘧啶掺入骨水泥后的释放及活性,评价其掺入骨水泥后经皮介入治疗小鼠移植瘤模型的抗肿瘤效应。材料和方法:采用洗提法和MTT法测量药物的释放及活性。60只昆明种小鼠,每只小鼠皮下注射,S180肉瘤细胞1×106,7天后随机分为4组,每组15只。3组为治疗组,每一组各以上述一种抗癌药骨水泥0.5ml瘤块内注入;另1组为对照组,仅注入单纯骨水泥0.5ml。记录每组10只荷瘤小鼠肿瘤大小变化,余5只作病理检查。结果:三种药物均可从骨水泥中释放并具有杀伤肿瘤细胞效应;3组治疗组中,肿瘤体积在1～10天内逐日缩小或不增大;而对照组中肿瘤体积持续增大。病理检查见治疗组水泥块周边肿瘤组织片状坏死。结论:骨水泥携带常用抗癌药具有抗肿瘤效应。
Purpose： To determine the release of mitomycin C, vincristine sulfate and 5-flauouracil mixed with bone cement and the anticancer activity of the release drugs. The anti-cancer effects of them by percutaneous interventional technique were evaluated. Materials and Methods： The release of drugs was studied by elution test. The anti-cancer activity of the drugs was assessed by MTT method, 1×10^6 S180 cells was injected subcutaneously into 60 mice each. Seven days later, the mice were divided into 4 groups randomly. In three treatment groups, each anti-cancer agent bone cement 0.5ml was injected into tumor tissue of every mouse, respectively. The change of tumor size and the pathological examination were observed. Results： The release of those drugs could be detected within 10 - 24 days and their anti-cancer activity lasted about 12 or 24 hours on MTT method. The more volume of those drugs mixed with bone cement was, the more the release and anti-cancer activity was. In three treatment groups, the tumor size reduced gradually in 10 days. But still kepted on enlarging in control group. The tumor tissue around anti-cancer agent bone cement presented necrosis on the pathological examination. Conclusion： Those drugs released from bone cement have the anti-cancer effect.
出处
《中国医学影像学杂志》
CSCD
2007年第4期273-275,共3页
Chinese Journal of Medical Imaging
关键词
肿瘤
介入
丝裂霉素
长春新碱
尿嘧啶
骨水泥
MTT
mitomycin
vincristine
uracil
bone cement
MTT
interventional Technique
tumor
