摘要
目的探讨NucliSens HIV-1 QT试剂盒用于定量测定HIV-1感染者人群精液或精浆中HIV-1的可行性。方法在正常人精液、精浆和血浆中分别添加5个滴度的HIV-1RNA,用NucliSens HIV-1QT测定,观察精液和精浆成分对测定结果有无影响,进一步测定、分析15名HIV-1感染者血浆和精浆中的HIV-1病毒载量。结果发现精液中因含有严重抑制核酸扩增的现象故不能使用NucliSens HIV-1QT,但精浆中未见该抑制现象。用Nu-cliSens HIV-1QT测定分别添加HIV-1RNA的正常人血浆和精浆样本,结果之间差异无统计学意义;所测定的10份正常人精浆样本,未发现假阳性结果。在15名HIV-1感染者中,血浆和精浆的HIV-1检出率分别为80%(12/15)和40%(6/15),病毒载量范围分别为(<250-140000)cp/ml和(<250-46000)cp/ml。结论NucliSens HIV-1QT可用于定量测定HIV-1感染者人群精浆中的HIV-1,精浆和血浆中HIV-1病毒载量之间具有一定的相关性。
Objective To evaluate NucliSens HIV-1 QT（bioMerieux, Netherlands） in quantitating human immunodeficiency virus type 1 （HIV-1） RNA in whole semen or seminal plasma from HIV-1-infected people . Methods Five levels of HIV-1 RNA were spiked to whole semen, seminal plasma and blood plasma samples from healthy people, and then measured by NucliSens HIV-1 QT. The same method was used to measure HIV-1 RNA in seminal plasma and blood plasma samples from 15 HIV-1 infected people. Results Nucleic acid amplification inhibitors were found in whole semen but not in seminal plasma when using NucliSens HIV-1 QT. No significant difference was found between normal seminal plasma and blood plasma samples spiked with HIV-1 RNA, and no false positive result was found in 10 normal seminal plasma samples. For 15 cases of HIV-1 infection, HIV-1 was detected in 80% （12/ 15） of the plasma samples and 40% （6/15） of the seminal plasma samples, with viral loads of 〈250 to 140,000 cp/ ml and 〈250 to 46,000 cp/ml, respectively. Conclusions NucliSens HIV-1 QT could be used for quantitation of HIV-1 RNA in seminal plasma from HIV-1-infected people in China, and the viral loads in seminal plasma were corre- lated with those in blood plasma .
出处
《中国输血杂志》
CAS
CSCD
2007年第3期194-197,共4页
Chinese Journal of Blood Transfusion
