摘要
目的研究GPI-CD80融合蛋白对肿瘤生长的抑制作用及其机制。方法将纯化的GPI-CD80蛋白与HepG2细胞共培养后用丝裂霉素灭活,制成肿瘤疫苗。设单纯丝裂霉素灭活的HepG2细胞为对照疫苗。将两组疫苗与裸鼠脾淋巴细胞共培养,MTT法检测淋巴细胞增殖及细胞因子IL-2、IFN-γ量的变化;乳酸脱氢酶法检测CTL的活性变化;将两组疫苗接种于荷瘤裸鼠,测量裸鼠肿瘤体积的变化。结果脾淋巴细胞经肿瘤疫苗刺激后,OD值、IL-2、IFN-γ的量和CTL的活性均高于对照疫苗组,差异有统计学意义;实验组荷瘤裸鼠平均肿瘤体积小于阴性对照组和空白对照组,差异有统计学意义。结论GPI-CD80融合蛋白能够抑制荷瘤裸鼠瘤体的生长速度,其机制可能与诱导T淋巴细胞增生、刺激细胞因子IL-2、IFN-γ的分泌、增强CTL活性等有关。
Objective To study the antitumor effect GPI-CD80 fusion protein and its mechanisms. Methods A tumor vaccine was prepared by culturing HepG2 cells in the presence of purified GPI-CD80 followed by inactivation with mitomycin, with mitomycin-inactivated HepG2 cells as the control group. The two preparations were co-cultured with nude mouse splenic lymphocytes, and the changes of lymphocyte proliferation and the production of interleukin-2 （IL-2） and interferon-γ （IFN-γ） were detected by MTT assay. The cytotoxic T-lymphocyte （CTL） activity was evaluated by LDH-release assay, and the changes of gross tumor volume were measured in tumor-bearing nude mice after administration of different vaccines. Results The application of GPI-CD80 tumor vaccine resulted in significantly increased optical density, IL-2 and IFN-γ levels and CTL activity of the nude mouse splenic lymphocytes in comparison with the control groups. The average tumor volume in nude mice treated with GPI-CD80 tumor vaccine was significantly smaller than that in negative control and blank control groups. Conclusion GPI-CD80 fusion protein may inhibit the tumor growth velocity in nude mice, possibly by promoting lymphocyte proliferation, stimulating the production of the cytokines IL-2 and IFN-γ, and enhancing of CTL activity.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第7期1027-1029,共3页
Journal of Southern Medical University
基金
广州市科技计划项目(2004Z3-E4091)
广东省科技计划(2005B31201008)~~
关键词
GPI
CD80
肿瘤疫苗
glycosyl phosphatidyl inositol
CD80
tumor vaccine
