摘要
通过正交试验,研究了不同酶种类、底物浓度、酶浓度和水解时间对水解猪血红蛋白制备血红素的影响,结果表明:碱性蛋白酶是制备血红素的最佳酶,最优组合是:碱性蛋白酶+底物浓度4%+酶浓度2000U/g+水解时间10h。
Effects of different substrate concentration, enzyme concentration and hydrolysis time on haemachrome preparation from porcine hemoglobin were studied by the orthogonal experiments. The results showed： alkaline protease was the best enzyme for obtaining hemochrome, the best conditions for haemachrome preparation was alkaline protease ＋ substrate concentration of 4% ＋ enzyme concentration of 2000U/g ＋ hydrolyzing for 10h.
出处
《江西农业学报》
CAS
2007年第7期92-93,96,共3页
Acta Agriculturae Jiangxi
基金
北京市教委产学研合作项目和国家985支持项目
关键词
蛋白酶
血红素
猪血红蛋白
水解
Protease
Haemachrome
Porcine hemoglobin
Hydrolysis
