摘要
目的探讨人类白细胞抗原 G(HLA-G)蛋白在胎盘组织中的表达及其基因多态性与妊娠肝内胆汁淤积症(ICP)发病的关系。方法选取2004年4月至10月间,在四川大学华西第二医院产科及四川省妇幼保健院产科行剖宫产分娩的晚孕妇女60例,其中 ICP 孕妇30例(ICP 组),根据妊娠期是否接受过地塞米松治疗又将 ICP 组分为治疗组(15例)和非治疗组(10例,因未治病例少);正常晚孕妇女30例为对照(根据检测指标不同分为对照1组,15例;对照2组,30例)。采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(sP)法,检测 HLA-G 蛋白在对照1组、治疗组及非治疗组孕妇胎盘组织中的表达情况(以平均吸光度值表示);同时采用聚合酶链反应-序列特异性引物(PCR-SSP)法,检测 HLA-G 基因外显子8上14 bp 缺失多态性在 ICP 组及对照2组孕妇外周血及新生儿脐血中的分布。结果 (1)非治疗组胎盘组织中,HLA-G 蛋白的平均吸光度值为56±8,明显低于对照1组的70±10及治疗组的66±9,分别比较,差异均有统计学意义(P<0.05);治疗组与对照1组胎盘组织中 HLA-G 蛋白的平均吸光度值比较,差异无统计学意义(P>0.05)。(2)HLA-G 基因外显子8上14 bp 缺失多态性检测结果显示,对照2组及 ICP 组母儿的等位基因频率及基因型频率比较,差异均无统计学意义(P>0.05)。结论 ICP 患者胎盘组织中 HLA-G 蛋白表达下调可能是 ICP 发病机制之一;地塞米松对胎盘组织中 HLA-G 蛋白的表达具有上调作用;HLA-G 基因外显子8上14 bp 缺失多态性可能与 ICP 的发病无关。
Objective To investigate the expression of human leucocyte antigen G （HLA-G） on human placenta and its gene polymorphism in relation to intrahepatic cholestasis of pregnancy （ICP）. Methods Immunohistochemistry was utilized to detect the HLA-G protein expression on third trimester placenta of fifteen normal pregnant women （control group 1 ）, fifteen ICP patients treated with dexamethasone （ICP group with dexamethasone treatment ） and ten ICP patients treated without dexamethasone（ ICP group without dexamethasone treatment）. We used polymerase chain reaction with sequence-specific primer （PCR-SSP） method to detect the 14 bp deletion polymorphism in exon 8 of HLA-G gene of thirty normal pregnant women and their babies （control group 2 ）, thirty 1CP patients and their babies（ ICP group ）. Results （1） The positive expression of HLA-G on placenta extravillous cytotrophoblast and its mean optical density of ICP group without dexamethasone treatment 56±8 was significantly lower than those of normal control group 70±10 and ICP group with dexamethasone treatment 66±9 （P〈0.05）. No significant differences were found between normal control group and ICP group with dexamethasone treatment（P〉0.05）. （2） There were no statistical differences between the control group and the ICP group with regard to the allele and genotype of the 14 bp deletion polymorphism in exon 8 of
HLA-G gene （ P〉0.05 ）. Conclusions The reduced expression of HLA-G on placenta in ICP patients may alter the maternal-fetal immune response and thus be involved in the pathogenesis of this disorder. Dexamethasone can upregulate the expression of HLA-G on placenta. The 14 bp deletion polymorphism in exon 8 of HLA-G gene might not have a significant influence on the development of ICP.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2007年第7期443-447,共5页
Chinese Journal of Obstetrics and Gynecology
基金
高等学校博士学科点专项科研基金(20020610030)
