摘要
目的检测生长激素调素在幽门螺杆菌(Hp)阳性的慢性胃炎患者胃窦黏膜组织中的表达。方法随机选择门诊慢性胃炎患者18例,取胃窦黏膜组织,以快速尿素酶及改良Giemsa染色两种方法判断Hp感染。以逆转录聚合酶链式反应(RT-PCR)法检测标本中生长激素调素mRNA的表达。结果18例患者中,Hp阳性者10例,阴性者8例;10例Hp阳性患者中7例呈现生长激素调素的高表达,8例Hp阴性患者中仅3例呈现生长激素调素的低表达(P=0.004)。结论生长激素调素在Hp阳性的慢性胃炎患者胃窦黏膜中呈高表达,提示生长激素调素可能是介导Hp阳性的慢性胃炎患者胃肠功能紊乱的原因之一。
Objective To detect the expression of ghrelin in the antrum mucosa of Helicobacter pylori（Hp） positive patients with chronic gastritis. Methods The gastric antrum mucosa specimens of Hp positive （ n = 10） and Hp negative （ n=8） patients with chronic gastritis were obtained by gastroscopy. The presence of Hp was assured by rapid urease test and histology. Expression of ghrelin mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）. ResuLts Among 18 cases,Hp positive was in 10 cases,Hp negative was in 8 cases. Among 10 Hp positive cases, 7 cases had a high level mRNA of ghrelin. However,a low level mRNA of ghrelin was found in 3 cases among 8 Hp negative cases（ P =0. 004）. Conclusion The expression of a high level ghrelin mRNA was found in the antrum mucosa of Hp-positive patients with chronic gastritis, which suggested that ghrelin plays a role in gastrointestinal disfunction in the patients with chronic gastritis.
出处
《临床荟萃》
CAS
北大核心
2007年第14期992-993,共2页
Clinical Focus
