摘要
目的:通过检测络气虚滞大鼠动脉组织中血红素加氧酶/一氧化碳(HO/CO)系统活性的变化,探讨不同通络方药对血管的保护机制。方法:健康雄性Wistar大鼠随机分为对照组、络气虚滞组、人参组、双参组、通心络组,每组24只。透射电镜下观察动脉组织内皮细胞超微结构,免疫组织化学染色方法观察各组动脉组织中HO-1的表达,RT-PCR及Western印迹方法检测HO-1mRNA及其蛋白的表达,同时检测动脉组织中CO含量的变化。结果:超微结果显示,络气虚滞组动物血管内皮细胞游离面微绒毛显著减少,吞饮小泡数量亦显著减少,线粒体大部分嵴和膜融合甚至消失,粗面内质网严重脱颗粒。免疫组织化学染色结果显示:与对照组比较,络气虚滞组动脉组织中HO-1蛋白表达阳性信号明显减弱,3种通络方药可明显增强HO-1表达。RT-PCR及Western印迹检测结果显示,与对照组比较络气虚滞组HO-1mRNA及其蛋白表达明显下调(P<0.01,P<0.001),3种通络方药可使HO-1mRNA及其蛋白的表达明显增强(P<0.001),其中通心络组优于人参和双参组(P<0.001),同时通心络可使CO的释放量明显增加。结论:通络方药可通过增加内源性CO发挥细胞保护作用,该作用可能与其改善或修复HO系统、提高HO-1表达有关,这可能是通络方药保护血管组织免遭损伤的机制之一。
Objective：To investigate the vascular protective mechanism of different levels of herbs through detecting activity of HO-1/CO system in arterial tissues of rats with stagnancy of collateral-Qi in deficiency condition. Methods： Healthy male Wistar rats of cleanness level were randomly divided into control group, stagnancy of collateral-Qi in a deficiency condition group （SCQDC group）, Renshen group, Shuangshen group, and Tongxinluo group. The ultrastructure of endothelium cells in the artery was observed. RT-PCR, Western blot, and immunohistochemical approaches were used to detect JNK, c-Jun mRNA and albumen expression in the arterial tissues. Meanwhile, the content of CO in the arterial tissue was also detected. Results： In SCQDC group, the counts of pinocytotic cells and microvili on the free surface of vascular endothelial cells were significantly decreased; most crests of mitochondria fused with membrane, some even disappeared; serious degranulation was observed in the rough surfaced endoplasmic reticulum. Immunohistochemistry showed that the HO-1 positive signals in the arterial histiocyte weakened significantly compared with the control group. The HO-1 expression was increased in the 3 treatment groups. Compared with control group, SCQDC group had decreased HO-1 mRNA and albumen expression（P〈0.01, P〈0.001 ）. The 3 different kinds of Herbs to dredge collaterals increased the HO-1 mRNA and albumen expression to different degrees （P〈0. 001）. The increase of albumen in the Tongxinluo group was more than those in the Shuangshen group and the Renshen group（P〈0. 001）. Tongxinluo also significantly increased the CO content. Conclusion： Herbs to dredge collaterals can induce CO production by ameliorating or recovering the HO System, which may be one of the mechanisms for protecting the vascular tissues.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2007年第7期782-785,共4页
Academic Journal of Second Military Medical University
基金
国家重点基础研究发展计划(973计划)(2005CB523301).~~
关键词
络气虚滞
血红素加氧酶
一氧化碳
通络方药
stagnancy of collateral-Qi in deficiency condition
heme oxygenase
carbon monoxide
herbs to dredge collaterals
