摘要
目的探讨n-3多不饱和脂肪酸（Eicosapentaenoic acid EPA,Docosahexenoic acid DHA）对人单核细胞炎症因子——一氧化氮（NO）的分泌和诱导型NO合酶（iNOS mRNA）表达的影响,以及EPA、DHA对核转录因子（NFκB）与DNA结合活性的影响。方法硝酸还原酶法测定NO的含量,RT-PCR技术分析iNOS mRNA表达水平,凝胶电泳迁移实验检测NFκB与DNA的结合活性。结果EPA、DHA在10-20μg/ml浓度范围内能够降低体外培养的人外周血单核细胞NO的分泌和iNOS mRNA的表达并降低NFκB的DNA结合活性。结论EPA,DHA能够抑制单核细胞炎症因子NO的产生,减少iNOS mRNA的表达,并通过抑制信号转导途径中NFκB与DNA的结合活性这一通路来抑制炎症因子分泌,产生抑制炎症作用。
Objective To study the effect of n-3 polyunsaturated fatty acids（EPA, DHA） on the production of NO, expression of iNOS mRNA and DNA-binding activity of NFκB in human monocyte. Methods RT-PCR and enzyme reduction method were used to measure the expression of iNOS mRNA and production of NO respectively. The DNA- binding activity of NFκB was assessed by EMSA. Results EPA, DHA can decrease the production of NO, expression ofiNOS mRNA and DNA-binding activity of NFκB between the dosage of 10μg/ml and 20μg/ml in human peripheral blood monocyte in vitro. Conclusion EPA, DHA have inhibitory effects on NO production, iNOS mRNA expression and DNAbinding activity of NFκB in monocyte. It indicates EPA, DHA suppress the production of inflammatory mediators via the NFκB signal transduction pathways in human monocyte, accordingly suppress inflammatory responses.
出处
《卫生研究》
CAS
CSCD
北大核心
2007年第4期445-448,共4页
Journal of Hygiene Research
