摘要
地菍叶片离体培养研究结果表明:诱导愈伤组织最佳的培养基为MS+1mg·L-12,4-D+0·2mg·L-16-BA+5%蔗糖+0·6%琼脂和MS+2mg·L-12,4-D+0·2mg·L-16-BA+5%蔗糖+0·6%琼脂;丛生芽分化增殖最佳培养基为MS+2mg·L-16-BA+0·3mg·L-1NAA+5%蔗糖+0·6%琼脂;生根最佳培养基为1/2MS+1·5mg·L-1NAA+0·5mg·L-12,4-D+5%蔗糖+0.6%琼脂和MS+0·5mg·L-16-BA+2mg·L-1NAA+5%蔗糖+0·6%琼脂。栽培观赏研究表明:盆栽最佳温度为28～32℃;最佳基质为泥炭土+蛭石(2:1)和腐殖土+蛭石(2:1);坪栽最佳肥料配比为N:P:K=1·4:1·2:1,最佳土壤为腐殖土。
In vitro culture and plant regeneration of Melastoma dodecandrum by tissue culture using its blade were studied. The results showed that the optimum medium for callus induction is MS supplemented with 2, 4-D 1 mg ·L^-1, 6-BA 0.2 mg ·L^-1, 5% sucrose and 0. 6% agar , or MS with 2, 4-D 2 mg·L^-1, 6- BA 0. 2 mg ·L^-1 , 5% sucrose and 0. 6% agar. The optimum medium for the formation and growth of cluster buds is MS with 6-BA 2 mg ·L^-1 , NAA 0. 3 mg ·L^-1 , 5% sucrose and 0. 6% agar. The optimum medium for rooting is 1/2MSwithNAA 1.5 mg·L^-1, 2, 4-D0.5 mg ·L^-1, 5% sucrose and0.6% agar, or MS with 6-BA 0. 5 mg ·L^-1 , NAA 2 mg ·L^-1 , 5% sucrose and 0. 6% agar. The cultivation for ornamental of test-tube plantlets was also studied. The optimum temperature is 28 -32℃. The optimum medium is 2/3 peat soil and 1/3 vermiculite, or 2/3 humus and 1/3 vermiculite for pot cultivation. The best proportion of N, P and K is 1. d ： 1.2 ： 1. The optimum soil is humus. The test-tube plantlets can be cultivated as a pot flower and ornamental lawn with high ornamental value.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第3期787-790,共4页
Acta Horticulturae Sinica
关键词
地菍
离体培养
植株再生
栽培
观赏植物
Melastoma dodecandrum
In vitro culture
Regeneration
Cultivation
Ornamental
