摘要
目的:研究喜树叶片、茎段和带芽茎段的愈伤组织诱导效果以及愈伤组织继代培养条件。方法:以MS为基本培养基,附加不同质量浓度6-BA,NAA和2,4-D,通过正交试验研究愈伤组织继代培养的适宜条件。结果与结论:MS+6-BA2 mg.L-1+NAA2 mg.L-1+2,4-D 2 mg.L-1的诱导效果较好,容易诱导出愈伤组织,其中叶片的诱导率可达80%以上。愈伤组织在MS+6-BA1 mg.L-1+2,4-D 1 mg.L-1培养基上继代培养生长快速,疏松易分散,适合用于细胞培养。
Objective： To study the callus induction from leaf, stem segments and stem segments with axillary＇ s bud and the subculture conditions of callus in Camptotheca acuminata. Method： The explants were inoculated into the media of MS with different concentrations of 6-BA, NAA and 2, 4-D by orthogonal experiment. Result and Conclusion： The optimal medium for callus induction was MS with 6-BA 2 mg·L^-1, NAA 2 mg·L^-1 and 2,4-D 2 mg·L^-1. It had better effects in leaf than in other explants. The induction ratio in leaf reached above 80%. The callus subcultured on the medium of MS with 6-BA 1 mg·L^-1 and 2, 4-D 1 mg·L^-1 grew vigorous and more quickly than that in other media. It was loose, friable in consistency and suitable for cell culture.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第13期1273-1276,共4页
China Journal of Chinese Materia Medica
关键词
喜树
愈伤组织
组织培养
Camptotheca acuminata
callus
tissue culture
