摘要
目的观察钙结合蛋白S100A2在原发性肝癌中的表达及与肿瘤细胞增殖的关系,探讨两者对肝癌患者预后的影响。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶(SP)法检测62例肝癌标本中S100A2的表达,同时用计算机图象分析技术对其DJ值(DNA含量指数)进行检测。结果高/中分化组,无门脉/胆管浸润或淋巴结/远处转移的病例中,S100A2阳性表达率分别为77.27%、76.92%、80.00%;DI值分别为1.68±0.20、1.73±0.23、1.76±0.23;但随着肝癌分化程度的降低,出现门脉/胆管浸润或发生淋巴结/远处转移,S100A2阳性表达率降低(50.00%、47.22%、50.00%)(X^2=4.39、5.53、5.07,P均<0.05),而DI值明显升高(1.93±0.29、1.99±0.31、2.01±0.33)(t=3.612、3.794、3.379,P均<0.01)。并且在短/中期生存患者中,S100A2阳性表达率(50.00%)明显低于长期生存者(100%)(X^2=10.05,P<0.01),而DI值(1.88±0.25)却明显高于长期生存者(1.69±0.21)(t=2.606,P<0.05)。S100A2的表达与DI值呈明显负相关(t=3.023,P<0.05)。结论S100A2蛋白表达降低和高的DI值提示肝癌的低分化、高侵袭转移能力及预后不良,两者的联合检测对判断肝癌的恶性程度和预后具有重要的临床意义。
Objective To explore the expression of S100A2 in primary hepatocellular carcinoma （HCC） and its relation with proliferation of tumor cells. Methods S100A2 expression was detected by using S-P immunohistochemical staining and DNA content index （DI） was measured by Feulgen histochemical staining and image analysis. Results The down-expression of S100A2 and high DI were correlated with tumor poor differentiation, high invasive and metastatic potentials and poor prognosis （Χ^2 = 4.59, 5.53,5.07,10.05; t=3.612,3.794,3.379,2.606,P〈0.05 or P 〈0.01）. There was a strong striking association between the S100A2 expression and DI （Χ^2 = 3. 023, P 〈 0.05 ）. Conclusion The down-expression of S100A2 and high DI suggest lower differentiation, higher metastatic potentials and poor prognosis of HCC. Combined detection of them may be helpful to evaluate malignant degree of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第7期790-791,I0001,共3页
Chinese Journal of Experimental Surgery
关键词
癌
肝细胞
S100A2
增殖
Carcinoma
Hepatocellular
S100A2
Prohferation
