摘要
研究了以玉米为基质的灵芝固体发酵,通过单因素和正交试验对工艺条件进行了优化,在测定菌质多糖含量的同时,测定了菌质中还原糖、蛋白质、游离氨基酸的含量。结果表明:玉米粒度、菌种、接种量、发酵温度和时间对基料中菌质多糖的产生及主要成分含量有显著影响,发酵条件优化结果为10目大小的玉米基料,接入12%液体菌种,28℃发酵20d。经优化后,多糖含量可以达到21.97mg/g,还原糖、蛋白质、游离氨基酸含量也优于其它处理组。
The Production of Ganoderma luciclum by solid-state fermentation using corn as substrate was studied , and the fermentation technique was optimized by single factor and orthogonal tests. The content of polysaccharide,reducing sugar,protein and amino acid were determined.The results showed that the size of crush granule, strain, time, temperature and inoculum size had significant effect on the content of polysaccharide,reducing sugar,protein and amino acid. The optimum fermentation conditions were 10 mesh granularity, 12% inoculum size, 28℃ fermentation temperature, 20d fermentation time. The content of polysaccharide was 21.97 mg/g under the optimized cultivation.
出处
《中国农学通报》
CSCD
2007年第7期422-427,共6页
Chinese Agricultural Science Bulletin
关键词
灵芝
固体发酵
玉米
多糖
条件优化
Ganoderma lucidum, Solid-state fermentation, Corn, Optimum conditions
