摘要
目的探讨基因survivin、cyclin D1的表达与儿童急性白血病(AL)的关系。方法采用RT-PCR方法对42例初发或复发AL患儿和13例完全缓解AL(AL-CR)患儿及15例非恶性疾病患儿骨髓单个核细胞进行基因survivin、cyclinD1表达的检测。结果①AL组survivin、cyclinD1mRNA的阳性率分别为78.6%和42.9%,高于AL-CR组和对照组(P<0.01);完全缓解组和对照组差异无统计学意义(P<0.05);急性淋巴细胞白血病(ALL)组和急性非淋巴细胞白血病(ANLL)组之间表达差异无统计学意义。AL复发组cyclin D1 mRNA阳性率明显高于初发组。②儿童AL初发者survivin与cyclin D1的阳性表达差异无统计学意义。③cyclin D1 mRNA阳性者缓解率低。结论儿童AL中存在survivin、cyclin D1异常表达,两者阳性表达差异无统计学意义;cyclin D1高表达者可降低AL的化疗敏感性,cyclin D1对AL有判断疗效和预后的价值。
Objectives To investigate the gene expression of survivin, cyclin D1 in childhood acute leukemia （AL） . Methods The expression of survivin and cyclin D1 were determined by RT-PCR in bone marrow mononuclear cells of 42 cases of incipient or relapsed childhood AL and 13 cases of complete remission AL （AL-CR）. Fifteen children without malignant diseases were included as the control group. Results （1）The detection rate of survivin and cyclin D1 mRNA in AL group were 78.6% and 42.9%, respectively, which were significantly higher than those in the AL-CR group and the control group （P 〈 0.01 ）. There were no significant difference of survivin and cyclin D1 expression between ALCR group and control group （P 〉 0.05）. There were also no significant difference of survivin and cyclin D1 expression between acute lymphoblastic leukemia （ALL） group and acute non-lymphocytic leukemia （ANLL） group. The detection rate of cyclin D1 mRNA expression in the relapsed group is significantly higher than the incipient group. （2）There was no difference between the expression of survivin and cyclin D1 in the incipient AL group. （3）The percentage of complete remission in patients with cyclin D1 mRNA expression was lower than that in patients without cyclin D1 mRNA expression. Conclusions Survivin and cyclin D1 were abnormally expressed in childhood AL. There was no statistical significance in their difference in expression. The overexpression of cyclin D1 mRNA might reduce the patients＇ s sensitivity to chemotherapy. Therefore, detecion of cyclin D1 may be useful for determining the treatment efficiency and prognosis.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2007年第7期570-573,共4页
Journal of Clinical Pediatrics
