血浆雌二醇,孕酮与外周血白细胞雌激素受体的相关性研究

The study of correlativity between serum estradiol, progesterone and estrogen receptor of peripheral blood white cells

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摘要目的:探讨外周血白细胞雌激素受体（ER）在月经周期的变化规律以及与血浆雌二醇（E2）、孕酮（P）的相关性。方法：应用放射配体结合法测定了18例月经周期正常的育龄妇女外周血白细胞ER含量的变化并与血浆E2、P浓度变化进行相关性分析。结果：外周血白细胞ER含量与子宫内膜细胞ER含量及血浆E2浓度在月经周期中的变化规律一致。血浆E2对白细胞ER有正向调节作用，呈显著正相关（r＝0．789，P＜0．001）；血浆P对白细胞ER有反向调节作用，呈显著负相关（r＝－0．347，P＜0．01）。结论：血浆E2、P对外周血白细胞ER及子宫内膜细胞ER的调节规律一致；外周血白细胞ER测定可间接反映子宫内膜细胞ER的变化，具有临床应用价值。 To study the change of estrogen receptor (ER) content in human peripheral bloodwhite cells (PBWC) during different phases of menstrual cycle and the correlativity between concentrationsof serum estradiol (E,), progesterone (P) and ER content in PBWC. Methods: ER content in PBWC wasmeasured by radioligand binding assay in 18 fertile women with normal menstrual cycle. The analysis ofcorrelativity was carried out between concentrations of serum E,,P and ER content in PBWC during follic-ular, periovulation and mid-luteal phases. ResuIts:The change of ER content in PBWC in normal menstru-al cycle paralleled to ER c0ntent in endometrium ahd serum E, concentration. The capacity of ER in PBWCwas up-regulated by serum E,, and the correlativity between the capacity of ER in PBWC and serum E,was significantly positive (r= 0. 789,P<0. 001 ). In contrast,the capacity of ER in PBWC was down-regu-lated by serum P in luteal phase, and serum P was negatively correlated with ER content in PBWC(r== 0. 347, P<0. 0l ). Conclusion:The change of the capacity of ER in PBWC can significantly indicate the change of ER content in endometrium. The method for measurement of ER content in PBWC has been identified t be better than that in traditional way.