摘要
为探讨ＨＬＡ－ＤＲ和－ＤＱ等位基因与我国汉族类风湿关节炎（ＲＡ）的相关性，采用聚合酶链反应－限制性内切酶片段长度多态性分析（ＰＣＲ－ＲＦＬＰ）方法对汉族人群中３５例ＲＡ患者和１００名健康人的ＤＲ和ＤＱ位点进行ＤＮＡ定型分析。结果发现，ＤＲ４频率在正常人为２４．０％，在ＲＡ患者为５１．４％（Ｐ＜０．０１，ＲＲ＝３．３０）；ＤＲ４亚型位点分析发现２组均以ＤＲＢ１＊０４０５占多数，但ＤＲＢ１＊０４０５频率在ＲＡ组为３１．４％，高于正常人的１０．０％（Ｐ＜０．０１）。ＤＱ４频率在全部ＲＡ患者为３７．１％，在ＤＲ＋４ＲＡ患者为７２．２％，均高于全部正常人的１０．０％（Ｐ＜０．０１）和ＤＲ＋４正常人的４１．６％（Ｐ＝０．０３７６）；ＤＲ＋４－ＤＱ＋４ＲＡ患者的病情重于ＤＲ－４患者。结果提示，ＤＲ４、主要是ＤＲＢ１＊０４０５与ＲＡ相关，ＤＱ４可增加ＤＲ４对ＲＡ的易感性，ＤＲ＋４－ＤＱ＋４单倍型是ＲＡ病情严重程度的标志。
We analysed the associations of DR and DQ alleles with rheumatoid arthritis(RA) in Han nationality of China. PCR RFLP technique was used to determine DRB 1,DQA 1 and DQB 1 alleles in 35 unrelated patients with RA and 100 healthy controls from the Hans.The frequency of DR 4 was 51.4% in RA patients and 24.0% in the healthy controls ( P <0.01, RR =3.30).Subtyping of DR 4 revealed no differences in the relative distributions of the DR 4 alleles between the RA group and the controls,which both were predominated by DRB 1*0405 allele,but there was a significant increase in the absolute frequency of DRB 1*0405 in RA patients compared with the healthy controls (31.4% vs 12.0%, P <0.01). DQ 4 (DQB 1*04) was also increased both in the RA patients compared with the healthy controls ( 37.1% vs 10.0% , P <0.001) and in DR + 4 RA patients compared with DR + 4 healthy ones ( 72.2% vs 41.6% , P = 0.0376 ).Further analysis showed that the haplotype DR 4 DQ 4 was associated with RA ( RR = 5.17 , P < 0.001 ), and that the DR + 4 DQ + 4 patients had more severe disease than the DR - 4 patients. These results suggest that DR 4 , mainly the DRB 1*0405 ,is associated with RA in Han nationality,and DQ 4 may play a role in DR 4 conferring susceptibility to RA. The DR 4 DQ 4 haplotype could be taken as a predictive marker for disease severity.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1997年第4期234-237,共4页
Chinese Journal of Internal Medicine
