摘要
【目的】以烟草(NC-82)为试验对象,运用组织培养方法,通过对苯磺隆对烟草愈伤组织抗性发展的影响、对愈伤组织生长的影响、对愈伤组织分化率的影响、对愈伤组织乙酰乳酸合成酶活性的影响和抗性筛选再生烟草苗抗性潜势进行较系统的研究,以期对ALS抑制剂类除草剂的抗性研究和科学合理使用以延缓抗药性的发生,以及对作物的抗性育种提供一定的理论依据。【方法】将烟草诱导产生的胚性愈伤组织在含有不同剂量磺酰脲类除草剂苯磺隆的MS培养基上继代培养进行抗性诱导,并对愈伤组织的生长情况及其ALS对苯磺隆敏感性进行跟踪检测;对抗性筛选再生烟草苗进行抗性检测。【结果】随着培养代数的增加,愈伤组织对苯磺隆的抗药性逐渐增强,经过12代的培养,0、2、3、5a.i.mg·L-14个剂量下的抗性指数分别达到1.4、3.6、11.6、18.5,LC50分别为4.2、10.8、34.9、55.7a.i.mg·L-1,组织内乙酰乳酸合成酶(ALS)对苯磺隆的敏感性也逐渐降低。高剂量苯磺隆30a.i.g·ha-1茎叶处理抗性再生烟苗,4个剂量下的成活率分别为5.8%、27.3%、43.8%和63.3%。在培养过程中烟草愈伤组织的分化率逐渐降低,并对苯磺隆产生轻微抗药性,但与苯磺隆的诱导无密切关系。【结论】烟草愈伤组织对磺酰脲类除草剂产生抗药性的速率很快,且选择压越高,抗药性发展越快。苯磺隆显著抑制烟草愈伤组织的生长,在形态上也发生变化,较对照色泽略深,质地紧密,组织内乙酰乳酸合成酶(ALS)对苯磺隆的敏感性也逐渐降低;烟草愈伤组织苯磺隆产生的诱导抗性可以在烟草整株水平上表达,诱导抗性随着诱导剂量的增加而增强。
[ Objective ] Research was carried out to study the relationship of tribenuron-methyl with resistance development, embryonic callus growth, regeneration rate, ALS sensitivity, the resistance of regenerated plantlet, bring academic support to the study of resistance of ALS inhibitor herbicides and resistance breeding. [Method] Embryonic callus derived from tobacco （NC-82） were transferred to MS culture medium substrate with different doses of tribenuron-methyl and bred for 12 generations, [Result] The callus resistance to tribenuron-methyl was enhanced during subculture with resistance index of 1.4, 3.6, 11.6 and 18.5. LCs0 was 4.2, 10.8, 34.9, 55,7 a.i.mg.L^-1 respectively with selected doses 0, 2, 3, 5 a.i.mg.L^-1 at the 12th generation while ALS sensitivity to tribenuron-methyl was reduced. The speed of tobacco callus resistance development was faster with higher dose. The survival rates of regenerated plantlet treated with higher doses（30 a.i.g.ha^-1） of tribenuron-methyl were 5.8%, 27.3%, 43.8% and 63.3%, and the induced resistance was expressed in the plantlet. During subculture the regeneration rate of callus was reduced and engendered slight resistance, but not related with tribenuron-methyl inducement. [Conclusion] The resistance development of tobacco embryonic callus to tribenuron-methyl was very fast, with higher speed in the higher selective press. Tribenuron-methyl obviously restrained the callus growth, changed the callus appearance. At the same time ALS sensitivity to tribenuron-methyl was reduced, and the resistance was expressed in the regenerated plantlet.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第7期1410-1415,共6页
Scientia Agricultura Sinica
基金
山东农业大学博士基金
关键词
烟草
苯磺隆
抗性诱导
组织培养
Tobacco
Tribenuron-methyl
Resistance inducement
Tissue culture
