摘要
将带有编码β－半乳糖苷酶基因片段（ＬａｃＺ）的质粒ｐＳＶ－β－Ｇａｌａｃｔｏｓｉｄａｓｅ（以下均简称ｐＳＶ－β－Ｇａｌ），通过股四头肌接种小鼠，利用ＥＬＩＳＡ方法检测小鼠体内β－半乳糖苷酶获得表达后刺激机体产生抗β－半乳糖苷酶抗体的情况。结果证实，编码β－半乳糖苷酶的核酸可以在哺乳动物骨骼肌中表达β－半乳糖苷酶，进而刺激机体免疫系统产生免疫应答。
Inoculated β Galactosidase gene in pSV β Gal plasmid into the quadriceps muscle of mice.The β Galactosidase specific antibodies in the sera of immunized mice were determined by ELISA.Resultant data showed that Galactosidase encoding DNA in plasmid could be expressed in murine quadriceps muscle and the expressed Galactosidase was able to stimulate the immune system to produce specific antibodies.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1997年第3期146-147,149,共3页
Chinese Journal of Immunology
基金
国家教委跨世纪优秀人才基金
关键词
Β-半乳糖苷酶
核酸
免疫
β Galactosidase Nucleic acid Immunization
