摘要
运用激光共聚焦扫描技术,在p38MAP激酶专一抑制剂SB202190处理下,探索植物促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAP激酶)介导蚕豆(Viciafaba)保卫细胞中H2O2为代表的活性氧(reactive oxygen species,ROS)信号机制,发现:p38MAP激酶专一抑制剂SB202190处理没有导致蚕豆保卫细胞中H2O2和Ca2+探针荧光强度增强,与水杨酸(salicylic acid,SA)或脱落酸(abscisic acid,ABA)迅速加强2种探针荧光强度形成鲜明对比;而该抑制剂分别与SA和ABA共同处理,前者H2O2探针荧光强度没有增加,而后者荧光强度仍然能够增加;而进一步使用Ca2+螯合剂BAPTA和SB202190+SA共同处理,H2O2探针荧光强度没有增加。这些结果初步表明:无论胞质Ca2+浓度高低,SB202190调节蚕豆保卫细胞中SA诱导H2O2产生,但是不调节植物逆境信使分子ABA此类的反应。因此推测,植物细胞中可能有类似动物和酵母细胞中的p38MAP激酶类,并可能专一调节植物保卫细胞中H2O2信号通路。据我们所知,这是首次报道SB202190和SA共同调节植物保卫细胞中ROS信号过程。
Using laser scanning confocal microscopy, we tested the mechanism of mitogen-activated protein kinase （MAPK） regulation of salicylic acid （SA）- or abscisic acid （ABA）-induced reactive oxygen species H2O2 elevation in Vicia guard cells. SB202190, a special inhibitor of p38 MAPK, blocked the elevation of H2O2 by SA but not ABA, whereas SB202190 alone could not increase H2O2 or Ca2＋ level in guard cells. The combination of BAPTA, the chelator of Ca^2＋, and SA＋SB202190 could not increase H2O2 level, which suggests that activation of p38 MAPK-like especially modulates H2O2 signaling in plant cells, regardless of Ca^2＋ level, similar to the mechanism in mammalian or yeast cells. To our knowledge, this is the first report that SB202190 regulates SA- induced ROS signaling in plant cells.
出处
《植物学通报》
CSCD
北大核心
2007年第4期444-451,共8页
Chinese Bulletin of Botany
基金
国家自然科学基金(No.30470895)
河南省高校新世纪优秀人才支持计划(No.2005HANCET-06)
河南省青年骨干教师资助计划
