摘要
目的：观察氧化型低密度脂蛋白（ox-LDL）对大鼠腹腔巨噬细胞血凝素样氧化型低密度脂蛋白受体-1（LOX-1）表达的影响，探讨LOX-1在泡沫细胞形成中的作用。方法：无菌分离、培养大鼠腹腔巨噬细胞，分别以终浓度10mg／L，25mg／L，50mg／L，75mg／L，100mg／L ox—LDL与巨噬细胞共孵育24h，以RT—PCR和Western blotting检测LOX-1基因表达水平；以终浓度75mg／L ox—LDL与巨噬细胞分别共孵育0．5h，3h，6h，12h，24h和36h后检测LOX-1表达水平；以不同浓度辛伐他汀预处理巨噬细胞后，再与ox-LDL共培养，检测LOX-1基因表达。结果：ox-LDL终浓度为10—75mg／L时，随着浓度增大，LOX-1mRNA和蛋白表达均增强，75mg／L时达高峰，ox-LDL 100mg／L时LOX-1表达显著降低（均P〈0．05）；自ox-LDL与巨噬细胞共孵育6h开始，LOX-1表达逐渐增强，24h达高峰，36h时LOX-1表达下降（均P〈0．01）；不同浓度辛伐他汀均使LOX-1表达降低，与对照组比较差异有统计学意义（P〈0．05）。结论：ox-LDL在一定范围内以剂量和时间依赖方式增强大鼠腹腔巨噬细胞LOX-1表达，LOX-1在巨噬细胞摄入ox-LDL的过程中可能具重要作用，辛伐他汀可抑制巨噬细胞LOX-1表达。
Objective To investigate the effect of ox - LDL on the expression of LOX - 1 in cultured celiac macrophages of rats and to explore the role of LOX - 1 in foam cell formation. Methods Celiac macrophages of rats were collected sterilely and co - incubated with ox - LDL for 24hrs, which final concentrations were 10 mg/L, 25 mg/L, 50 mg/L, 75mg/L and 100 mg/L respectively. Total RNA and protein were extracted ; LOX - 1 expressions were measured by RT - PCR and Western blotting respectively. Macrophages were cultured with ox- LDL （75mg/L） for 0. 5hrs, 3hrs, 6hrs, 12hrs, 24hrs and 36hrs respectively, then total RNA and protein were extracted, expressions of LOX - 1 were measured by RT - PCR and Western blotting. Macrophages were pretreated with different dosages of Simvastatin and then co - incubated with ox - LDL; expressions of LOX - 1 were also measured. Results Expressions of LOX - 1 increased gradually as the con- centration of ox -LDL increased between 10 N 75mg/L, reached the peak when the concentration was 75mg/L, and decreased dramatically when it was 100mg/L. Compared with control group, the differences of changes were significant （ P 〈0.05, either）. After incubated with ox - LDL for 6hrs, the expressions of LOX - 1 began to increase gently and reach the peak at the 24hrs, and decreased after 36hrs. All the differences of changes were significant compared with control group （ P 〈0. 01 , either）. Expressions of LOX - 1 were depressed by different dosages of Simvastatin and the differences were significant compared with control group （ P 〈 0. 05, either）. Conclusion Ox - LDL increased the expression of LOX - 1 in celiac macrophages of rats in a dosage and time dependent manner. LOX - 1 may play a crucial role in the internalization of ox - LDL. Simvastatin can reduce the expression of LOX - 1.
出处
《中国医师杂志》
CAS
2007年第6期753-756,共4页
Journal of Chinese Physician
