摘要
目的:探讨益气活血解毒方对经皮血管腔内成形术(PTA)后再狭窄的防治作用。方法:采用电刺激兔颈总动脉加高脂饲料8周后,体表多普勒超声评价颈动脉粥样斑块狭窄,再行颈动脉PTA,形成再狭窄。造模后动物随机分为模型组、西药组(普伐他汀和巴米尔)、中药组(益气活血解毒方),另设正常组和假手术组作为对照,含药饲料治疗4周。分别在不同时间点检测血脂、血清C-反应蛋白(CRP)、转化生长因子(TGF-β)。药后4周颈总动脉取材,常规HE、弹力+VG和Masson染色,平滑肌肌动蛋白(α-SM-actin)和巨噬细胞RAM11免疫组化分析,所有病理切片经显微图像分析系统分析。结果:益气活血解毒方突出表现为对增生内膜中胶原的抑制作用,减少狭窄局部胶原面积比达50%;能减少血清中TGF-β的含量,降低血脂;而对血清CRP、增生内膜中巨噬细胞RAM11的表达无明显抑制作用。结论:益气活血解毒方通过减少增生内膜中胶原的堆积、降低血脂和TGF-β的含量,防治PTA后再狭窄。
Objective： To approach preventive and therapeutic effect of Yiqihuoxuejiedu decoction on restenosis. Methods： Model of artherosclerosis restenosis in rabbits was created by stimulating arteria carotis communis with electric current, feeding rabbits with high-fat diet for 8 weeks, Doppler ultrasound estimating stegnosis of atheromatous plaque, and then broadening stenosal carotid with sacculus in home position. Model rabbits were divided into three groups randomly, consisting of model, western medicine （pravastatin plus Bamyl）, Chinese drug （Yiqihuoxuejiedu decoction）. Normal and Sham operation group was negative control. Medicine containg fodder treated rabbits for 4 weeks. Blood-fat, C-reactive protein, transforming growth factor in the serum were detected respectively. HE, elasticity ＋ VG, Masson, and immuno-histochemical α-SM-actin and macrophage RAM11 were determined. All results of pathological dyeing were gained by image analysis. Results： Yiqihuoxuejiedu decoction had outstanding effect on inhibiting collagen in hyperplasy endomembrane. It reduced an area of collagen reaching 50% （compared with modal group P 〈 0.01 ）. Yiqihuoxuejiedu decoction also decreased the content of TGF-β and blood-fat in serum. However, Yiqihuoxuejiedu decoction had no effect on lowering content of CRP and inhibiting expression of macrophage RAM11. Conclusion：Yiqihuoxuejiedu decoction may prevent and treat restenosis after percutaneous transluminal anglography by diminishing collagen in hyperplasy endomembrane, depressing the content of blood-fat and transforming growth factor β.
出处
《中国实验方剂学杂志》
CAS
2007年第3期32-36,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药管理局资助课题(2000-J-p-21)
关键词
经皮血管腔内成形术
再狭窄
益气活血解毒方
胶原
转化生长因子Β
Percutaneous transluminal angiography
restenosis
Yiqihuoxuejiedu decoction
. collagen
transforming growth factor β
