摘要
目的:比较补肾活血复方中药与激素替代疗法对绝经后骨质疏松患者血清细胞因子水平的影响。方法:选择深圳市龙岗区南岭医院中医骨伤科收治的绝经1年以上的骨质疏松症女性65例。①实验分组:采用随机数字表法分为补肾活血复方中药组35例和激素替代治疗组30例。实验经医院伦理委员会审批,患者均知情同意。②实验方法:补肾活血复方中药组给予补肾活血方(成分为淫羊霍、鹿角胶、山萸肉、补骨脂、当归、白芍、熟地黄、牛膝、木瓜、何首乌、寄生、续断、水蛭、甘草等)煎服,2次/d;激素替代治疗组给予替勃龙片(利维爱),每天睡前服1次,有子宫者每个周期加服安宫黄体酮6mg/d,服用30d。③实验评估:测定细胞因子水平:取两组患者全血加入2mmol/L谷氨酰胺,100U青霉素,100mg/L链霉素,分布至2mL的孔中,一半孔不作处理为空白对照组;一半孔加入多克隆刺激剂5mg/L植物血凝素和25mg/L脂多糖为刺激组。采用ELISA法测定白细胞介素1β、白细胞介素6、肿瘤坏死因子α。结果:①两组白细胞介素6水平:补肾活血复方中药组低于激素替代治疗组,经多克隆刺激后也低于激素替代治疗组,差异均有显著性意义(P<0.05)。②两组白细胞介素1β、肿瘤坏死因子α水平:空白对照和经多克隆刺激后差异均无显著性意义(P>0.05)。③两组细胞因子相关分析:白细胞介素1β、白细胞介素6、肿瘤坏死因子α水平空白对照和经多克隆刺激后呈正相关,差异有显著性意义(r=0.7125,0.6587,0.8421,P<0.001)。结论:补肾活血复方中药治疗及激素替代治疗对血清白细胞介素1β和肿瘤坏死因子α的作用相近,补肾活血复方中药治疗对白细胞介素6的作用更强。
AIM： To investigate the curative effect of Compound traditional Chinese medicine （TCM） for invigorating kidney and promoting blood flow compared with the hormone replacement therapy （HRT） for serum cytokine level in postmenopausal osteoporosis patients. METHODS： A total of 65 cases with postmenopausal osteoporosis were recruited from the Department of Traditional Chinese Medicine and Bum Trauma in Nanling Hospital of Longgang District of Shenzhen City. （1）They were divided into TCM compound group （n =35） and HRT group （n =30）. The experiment was approved by the hospital ethnics committee, the patients were informed of and agreed with the treatment. （2）TCM compound prescription for invigorating kidney and promoting blood flow was consisted of longspur epimedium, deerhom glue, fructus comi, malaytea scurfpea fruit, Chinese angelica, white peony alba, prepared rehmannia root, achyranthes root, common floweringqince fruit, fleeceflower root, parasitism, Himalayan teasel root, leech, and radix glycyrrhizae, etc, two decoctions daily; HRT group was given oral administration of livial tibolone, once before retiring. Those remaining uterus were treated with 6 mg/d medroxy progesterone acetate for 30 days.（3）Whole blood samples obtained from two groups were added with 2 mmol/L glutamine, 100 U penicillin, 100 mg/L streptomycin. The 2-mL hole was assigned to two average sets, one was taken as blank controls while the other was taken as stimulation group, adding with 5 mg/L phytohemagglutinin and 25 mg/L lipopolysaccharide as multiclonal stimulator. Intedeukin （IL） 1β, IL-6, and tumor necrosis factor （TNF） α were detected using ELISA method. RESULTS： （1）Compared with HRT group, the level of IL-6 was significantly lower in TCM compound group and that after multiclonal stimulation （P 〈 0.05）. （2）There was insignificant difference in IL-1β and TNF-α levels between blank control group and multiclonal stimulation group （P 〉 0.05）.（3）The levels o
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第27期5421-5423,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
