摘要
目的:观察小鼠缺氧耐受对学习、记忆的影响。方法:40只昆明种小鼠,按体重随机分为正常对照组(12只),1次缺氧组和4次缺氧组(各14只)。分别采用Morris水迷宫法、跳台法和避暗法观察缺氧耐受对小鼠学习、记忆的影响。结果:水迷宫测试中,与正常对照组比较,1次缺氧组小鼠第1、2、4及5天的搜索平台的总时间均明显延长(P<0.05),第2、3及5天的总游程均明显增加(P<0.05);与1次缺氧组比较,4次缺氧组小鼠前5天搜索平台的总时间均明显缩短(P<0.01或P<0.05),第1、4及5天的总游程均明显缩短(P<0.01或P<0.05),第1天的朝向角明显缩小(P<0.05);与正常对照组比较,4次缺氧组小鼠第1天的总游程明显缩短(P<0.05)。跳台测试中,与正常对照组比较,1次缺氧组小鼠第2天错误次数明显增多(P<0.05);与1次缺氧组比较,4次缺氧组第2天的潜伏期和错误次数均明显减少(P<0.05)。避暗实验中,各组第2天的潜伏期和错误次数比较,差异均无显著性(P>0.05)。结论:1次缺氧可减弱小鼠的学习、记忆功能,缺氧耐受(4次缺氧)可减轻缺氧对学习、记忆的损害,甚至增强小鼠的学习、记忆能力。
Objective To study the effects of hypoxic tolerance on learning and memory in mice. Methods Forty mice were divided into 3 groups by weight randomly： control group, run 1 hypoxic group and run 4 hypoxic group. Morris water maze test, step-down test and step through test were used to observe the effect of hypoxic tolerance on learning and memory in mice . Results In the Morris water maze test, compared with control group, the total swimming time of run 1 hypoxic mice increased significantly on the 1st, 2nd, 4th, 5th day and the total swimming distance of run 1 hypoxic mice also increased evidently on the 2nd, 3th, 5th day （P〈0.05） ; compared with run 1 hypoxic mice, the total swimming time of run 4 hypoxic mice decreased significantly in the first five days （P〈 0.01 or P〈0.05）, and the total swimming distance of run 4 hypoxic mice decreased evidently on the 1st, 4th, 5th day （P〈0.01 or P〈0.05）, and the starting angle of run 4 hypoxic mice diminished evidently on the first day （P〈0. 05）; compared with control group, the total swimming distance of run 4 hypoxic mice decreased evidently on the first day （P〈0.05）. In the step-down test, compared with control group, wrong times on the 2nd day of run 1 hypoxic mice increased evidently （P〈0.05） ; compared with run 1 hypoxic mice, latency and wrong times on the 2nd day of run 4 hypoxic mice also decreased significantly （P〈0.05）. Conclusion Learning and memory of the mice injuried by run 1 hypoxic can be improved by run 4 hypoxic tolerance.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第3期470-473,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题(20030551-1)
关键词
缺氧
缺氧耐受
学习
记忆
小鼠
anoxia
hypoxic tolerance
learning
memory
mice
