摘要
对六种灵猫科物种线粒体12 S rRNA基因及其中四种的Cytb基因部分序列进行了测定,并从Gen-Bank获得斑林狸(Prionodon pardicolor)、熊狸(Arctictis binturong)的Cytb基因同源序列。两基因整合序列比对后长755 bp,12 S rRNA基因序列中有70个变异位点,31个简约信息位点,在Cytb基因序列中,共有120个位点呈现变异,60个简约信息位点,Cytb基因的碱基变异百分比高于12 S rRNA基因的碱基变异百分比。使用邻接法(NJ)、最大似然法(ML)重建的分子系统树显示:斑林狸从灵猫亚科中分离出来,支持灵猫亚科的多系起源,而且斑林狸可能是中国起源最早且最特化的灵猫科动物。另外,同属于灵猫亚科的大灵猫(Viverra zibe-tha)、小灵猫(Viverricula indica)聚为一支,同属于棕榈狸亚科的果子狸(Viverricula indica)、熊狸聚为姐妹群,这些与传统形态学分类观点一致。
Partial sequences of mitochondrial 12 S rRNA gene from 6 civets and Cyt b gene sequences from 4 species were sequenced. The combined data was 755 bp long, containing 191 variable sites, 90 parsimony informative sites. There were 120 variable sites in Cyt b gene sequence (375 bp), and it showed higher nucleotide variation (32 % ) than 12 S rRNA gene (370 bp), which only had 70 variable sites ( 18.4 % ). Molecular phylogenetic tree ( NJ and ML) supported that Viverrine was polyphyletic, and implied spotted linsang ( Prionodon pardicolor) perhaps was basal and mostly specified to other Viverridae species in China. Meanwhile, large Indian civet ( Viverra zibetha) , small Indian civet ( Viverricula indica) clustered together, masked palm civet ( Viverricula indica), binturong (Arctictis binturong) clustered together and they constituted monophyletic group respectively. These were consistent with traditional taxonomy arrangement.
出处
《激光生物学报》
CAS
CSCD
2007年第3期349-354,共6页
Acta Laser Biology Sinica
基金
安徽省优秀青年基金(04043049)
安徽省学术与技术带头人专项基金
生物环境与生态安全安徽省高校省级重点实验室基金