摘要
RM206是与水稻抗白叶枯病基因Xa23紧密连锁的微卫星标记,应用该标记对含有Xa23基因的品种386(CBB23)、优良育种材料384、385和387,杂交种F1、F2及回交群体BC1、BC2进行多态性分析。结果表明,RM206的PCR扩增产物在4个亲本材料之间的多态性较好,这种多态性能在杂交后代群体F1、F2及回交群体BC1、BC2中得到保持,RM206扩增产物的显隐性带型之间的比率在杂交后代群体F1、F2及回交群体BC1、BC2中符合孟德尔分离定律。RM206标记在各育种群体中未出现偏分离现象。
RM206 is a closely-linked microsatellite marker to the bacterial blight （BB） resistant gene Xα23, and its polymorphism was analyzed in the parents：386 or CBB23 （Xα23 carrier）, elite breeding stocks （384, 384, 387）, and their relevant breeding populations F1, F2, and BC1, BC2. The results showed that RM206 had a good polymorphism among the 4 parents, this kind of polymorphism still existed in the breeding populations F1, F2 and BC1, BC2. In breeding populations -F1, F2, and BC1, BC2, the ratio of the RM206 PCR products accorded with the Mendel＇s law of segregation. RM206 didn＇t show segregation distortion in each breeding population.
出处
《广西农业生物科学》
CAS
CSCD
2007年第2期120-124,共5页
Journal of Guangxi Agricultural and Biological Science
基金
广西科学基金(桂科回0448005)
关键词
水稻
白叶枯病
微卫星标记
多态性分析
rice
bacterial blight
microsatellite marker
polymorphism analysis
