摘要
目的:比较紫杉醇脂质体与紫杉醇注射液对S180腹水瘤小鼠的肿瘤抑制效果。方法:采用逆向蒸发法及超声法制备紫杉醇脂质体,以激光粒度散射仪测定粒径,以反向高效液相法测定脂质体中紫杉醇药物含量。采用S180细胞昆明鼠腹腔内注射形成腹水瘤模型,肿瘤种植24h后随机分成4组,分别为空白对照组、空白脂质体组、紫杉醇注射液组、紫杉醇脂质体组。每组5只,其中两组分别以紫杉醇脂质体和紫杉醇注射液腹腔注射,其他2组分别给予生理盐水和空白脂质体腹腔注射,共给药4次,给药间隔72h。结果:紫杉醇脂质体平均粒径为(282.40±8.94)nm,药物包封率为91%。肿瘤种植14天后,空白对照组、空白脂质体组、紫杉醇注射液组小鼠形成大量腹水,腹膜、肝脏广泛转移;紫杉醇脂质体组仅形成少量腹水,腹膜、肝脏未见转移。结论:紫杉醇脂质体腹腔内给药可以明显抑制腹水形成,有效抑制腹腔转移。
Objective：To compare the antitumor effect of paclitaxel-loaded liposomes with paclitaxel on S180 ascites-tumour bearing mice. Methods：The paclitaxel-loaded liposomes were prepared by reverse evaporation and sonication techniques. The particle diameters were measured by dynamic light scattering （DLS） measurement and the entrapment efficiency was measured by the reverse-phase high-pressure liquid chromatograph （RP-HPLC） method. Twenty Kunming mice bearing ascites tumor xenografts were distributed into four groups at random：the control,empty-liposomes,free paclitaxel group and the paclitaxel-loaded liposomes. Treatments were started after 24 hours of implantation. Two groups bearing S180 xenograft were treated i.p. with 10 mg/kg of paclitaxel （free drug or liposome encapsulated）. The other two groups were given i.p. with saline or empty-liposomes. All the treatments were given 4 times and the interval was 72 hours. Results：The average diameter of the paclitaxel-loaded liposome was 282.4 ± 8.94 nm. The entrapment efficiency was 91%. There were resembly much ascites in the three groups of the control, empty-liposomes and free paclitaxel. While in the group of paclitaxel-loaded liposomes,there was little ascites. In addition,there were dramatically little metastasis in abdominal cavity with the group of the paclitaxel-loaded liposomes other than the remain three groups. Conclusion： Ascites and metastasis in abdominal cavity could be inhibited by paclitaxel-loaded liposomes to S180 ascites-tumour bearing mice remarkly.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第7期706-710,F0003,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅项目(H200349)
关键词
紫杉醇
脂质体
腹水
腹腔转移
paclitaxel
liposome
ascites
metastasis in abdominal cavity
