摘要
探讨细胞代数和密度对血管平滑肌细胞(vascular smooth muscle cell,VSMC)表型重塑能力的影响及机制,观察血清饥饿诱导的不同代数和密度的VSMC骨架的组构特征及收缩反应性,检测细胞骨架中收缩蛋白的含量和比例变化。结果发现,低代数(3代)、高密度的VSMC经血清饥饿诱导后易于形成束状、极性排列的应力纤维,乙酰胆碱(Ach)刺激可产生明显的收缩反应。Western印迹显示,3代高密度VSMC中,平滑肌22α(SM22α)在F-肌动蛋白中的组成比例及其在F-/G-肌动蛋白的含量之比明显高于8代细胞。结果提示,SM22α在F-肌动蛋白中的分布比例可能决定了应力纤维的排布方式,是细胞获得收缩性的主要调节因素,在VSMC表型重塑过程中具有重要意义。
To investigate the effects and mechanisms of cell passage number and density on phenotypic remodeling ability of vascular smooth muscle cells （VSMCs）, cytoskeleton organization and contractile ability in VSMCs at different passage number and density were observed, and the changes of content and ratio of contractile proteins in cytoskeleton were detected. The results showed that low passage （passage 3） VSMCs at high density following serum deprivation were tending to form bundled and polar stress fibres, and their contractile ability induced by acetylcholine （Ach） was significantly higher than that in other groups. Western blot displayed that relative amount of smooth muscle 22α （SM22α） in F-actin fraction and its ratio in F-/G-actin fraction in passage 3 VSMCs at high density were remarkably higher than that in VSMCs at passage 8. In conclusion, distribution of SM22α in F-actin fraction may determine the organization of stress fibres, which is a key factor for contraction ability of VSMCs. This protein may play an important role in VSMCs phenotypic remodeling.
出处
《细胞生物学杂志》
CSCD
2007年第3期420-424,共5页
Chinese Journal of Cell Biology
基金
国家科技部重大基础研究前期研究专项项目计划(No.2005CCA03100)
国家自然科学基金(No.30570661)
河北省自然科学基金(No.C2006000814)资助项目
