摘要
[目的]比较雄性大鼠吸入纳米二氧化钛(nm-TiO2)与微米二氧化钛(nm-TiO2)对其生精功能的影响并探讨其作用机制。[方法]45只雄性Wistar大鼠随机分为5组,分别为nm-TiO2100 mg/m3和300 mg/m3组,μm-TiO2100 mg/m3和300 mg/m3组和对照组。动物隔天在装有风扇扬尘的静式染毒柜中吸入染毒2 h,对照组不扬尘,只吸入空气。65 d后,测定附睾尾精子数量、活动度和畸形率,睾丸组织中乳酸脱氢酶同工酶-C4(LDH-C4)、琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)的活性、丙二醛(MDA)含量和血清中MDA、SOD及8-羟基脱氧鸟苷(8-OHdG)水平。[结果]nm-TiO2致大鼠精子数量下降、LDH-C4活力下降和8-OHdG水平增加程度显著高于同剂量μm-TiO2组(P<0.05);nm-TiO2可致大鼠精子活动度、睾丸组织SDH和SOD活力下降,精子畸形率和MDA生成量升高(P<0.05),但与同剂量μm-TiO2组比较无显著性差异(P>0.05)。[结论]nm-TiO2和μm-TiO2对雄性大鼠生精功能影响存在差异,nm-TiO2致生殖损伤能力强于同剂量水平的μm-TiO2。
[Objective]To compare the effects of nanosized and microsized titanium dioxide on spermatogenesis function of male rats exposed by inhalation and explore their mechanism. [Methods]45 male rats were randomly divided into control group and four experimental groups which were exposed by 100 mg/m^3 or 300 mg/m^3 nanosized and microsized titanium dioxide in inhalation chambers for 2 hours every other day. Age-matched rats were exposed to room air with the same condition and served as controls. 65 days later, the quantity and quality of sperm, testicular Lactate dehydrogenase isoenzyme-C4 （LDH-C4）, Succinate dehydrogenase （SDH）, Superoxide dismutase （SOD） activities and Malondialdehyde （MDA） levels, serum SOD activity, MDA were examined. The changes of biochemical parameters in serum and testes were also measured. [Results]Nanosized titanium dioxide could reduce the sperm count of rats and the testicular LDH-C4 activitites, increase MDA levels in the testes and the effect was higher than that of microsized particles at the same concentration. Nanosized titanium dioxide could lead to the reduction of sperm motility, testicular LDH-C4 activitites and 8- hydroxydeoxyguanosine（8-OHdG） concentration in serum of particles-exposed rats compared with the control animals, but there was no significant difference compared with that of microsized particles at the same concentration. [Conclusion]The present findings suggestted a different effect of impairment of sperm production and maturation induced by inhalation of nanosized and microsized titanium dioxide, and microsized titanium dioxide exerted more severe reaction.
出处
《预防医学论坛》
2007年第2期137-140,共4页
Preventive Medicine Tribune
关键词
二氧化钛
纳米
微米
精子
睾丸
Titanium dioxide
Nanosized
Microsized
Sperm
Testis
