摘要
目的研究3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮(3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone,MX)对小鼠多组织的ras与野生型p53基因(wt-p53)表达的影响,从基因水平探讨MX致癌的机制。方法昆明种雄性成年小鼠,每天按21mgMX/kg的剂量经腹腔注射染毒,连续6d,生理盐水作溶剂对照。末次染毒24h后处死。运用原位杂交技术检测MX对小鼠肝、肾和小肠组织的ras基因与wt-p53基因表达的影响。结果MX染毒组小鼠的肝、肾和小肠的ras-mRNA表达水平(0.165±0.007,0.155±0.011,0.196±0.035)明显高于溶剂对照组(0.147±0.007,0.136±0.004,0.132±0.026),且差异有统计学意义(P<0.05)。wt-p53-mRNA表达水平在MX染毒组略为降低,但与溶剂对照组相比没有统计学意义。结论MX可诱导小鼠肝、肾和小肠组织中ras基因转录活性增强,但未能诱导野生型p53基因的异常表达。
Objective To investigate the effect of 3-chloro-4-dichloromethyl-5-hydroxy-2（5H）-furanone （MX） on the expression of ras and wt-p53 gene in mice, and to explore the possible mechanisms involved in MX-induced carcinogenesis. Method Male Kunming mice were treated with MX （21 mg/kg, i.p.） for six consecutive days. Normal saline （NaCl, 0.9% ） was used as solvent control. Mice were sacrificed at 24h after the last treatment. The expression of ras and wt-p53 genes in the liver, kidney and small intestine of mice was examined by in situ hybridization. Result The expression levels of ras-mRNA in liver, kidney and small intestine of MX-treated group （0.165±0.007, 0.155 ±0.011, 0.196±0.035 ） were significantly higher than their corresponding solvent control groups （0.147±0.007, 0.136±0.004, 0.132±0.026）. Although the expression level of wt-p53 mRNA was slightly decreased in MX-treated mice, the difference was not significant. Conclusion MX was found to increase the transcription of ras in liver, kidney and small intestine.
出处
《热带医学杂志》
CAS
2007年第6期505-508,共4页
Journal of Tropical Medicine
基金
国家自然科学基金(No.30170794)
