摘要
目的:评价体外创面模型环境中,釉基质蛋白对牙周膜细胞、牙龈成纤维细胞覆盖创面能力的影响。方法:利用在盖玻片上形成的人牙周膜和牙龈成纤维细胞的融合培养物,以机械方法去除7mm宽的细胞层条带,形成体外创面模型。受损培养物在含有10%胎牛血清的培养液中继续培养2、6、9d,同时以100μg/ml的釉基质蛋白分别刺激牙周膜、牙龈成纤维细胞,并与不加釉基质蛋白者作对照。玻片经固定后作甲紫染色。以计算机辅助图像分析系统对细胞覆盖体外创面的面积进行百分比定量,采用SAS6.12软件包进行样本均数的t检验。结果:对照组组内牙周膜、牙龈成纤维细胞覆盖创面的面积存在差异,牙龈成纤维细胞覆盖创面面积在创面形成后第9天显著高于牙周膜细胞,差异有显著性(P<0.05)。在加用100μg/ml釉基质蛋白的条件下,牙周膜细胞覆盖体外创面的面积较对照组明显增加,创面形成后第6、9天,牙周膜、牙龈成纤维细胞覆盖体外创面面积均无显著差异(P>0.05)。结论:牙龈成纤维细胞覆盖创面的能力高于牙周膜细胞。釉基质蛋白有增进创面愈合,特别是牙周膜细胞创面覆盖的作用。
PURPOSE： The aim of this study was to evaluate the influence of enamel matrix protein （EMP） on wound filling of periodontal ligament cells （PDLC） and gingival fibroblasts （GF）, using an in vitro model of wound healing, METHODS： In vitro wound models were mechanically created in subconfluent cultures of human GF and PDLC, by removing a 7mm wide band of the cell layer respectively, Wounded cultures were then incubated for a time periods up to 2,6,9 days in a media containing 10% fetal bovine serum （FBS） , stimulated with EMP （100μg/ml） simultaneously,negative controls were those cultured only with media containing 10% FBS. Slides were fixed, stained with crystal violet and cell filling area within the wound boundaries was quantified by computer assisted histomorphometry. Statistical analysis was performed by SAS6.12 software package to determine the differences between the time points and groups. RESULTS： in the control group, there was difference between GF and PDLC in filling wound in vitro over 9 days of healing period. The difference was significant （P〈0.05） at the 9th day after wound creating, with GF filling the wound faster than PDLC. In contrast, under the stimulation of EMP （100μg/ml）, there was no significant difference （P〉0.05） between the filling rate of two types of periodontal cells at the 6th, 9th day after wound creating. CONCLUSION： GF has a significantly greater ability to fill a wound than PDLC. However, EMP appears to exert an influence on cells that is compatible with improved wound healing, especially in that of PDLC. Supported by Research Fund of Shanghai Municipal Education Commission （Grant No.04BB25）,
出处
《上海口腔医学》
CAS
CSCD
2007年第3期272-276,共5页
Shanghai Journal of Stomatology
基金
上海市教育委员会科研基金(04BB25)
关键词
釉基质蛋白
创面愈合
模型
牙周膜
牙龈
Enamel matrix protein
Wound healing
Model
Periodontal ligament
Gingiva
