摘要
目的：探讨安迪（Adi）注射剂对人白血病K562细胞株增殖和凋亡的影响．方法：MTT法测定K562在Adi作用后的细胞活力（A值）；流式细胞技术检测细胞凋亡变化；电镜观察细胞的形态学改变等；综合评价Adi对K562细胞株增殖和凋亡的影响．结果：①MTT法测得Adi在（2．5～20．0）μg/mL浓度范围内能明显抑制K562细胞的增殖，并具有浓度依赖性，20．0μg／mL Adi作用36h的K562细胞株A值最低；②流式细胞仪检测结果显示10．0，20．0μg／mLAdi处理组K562细胞的凋亡率分别为50％和39％，明显高于对照组的10％（P〈0．05）；③电镜下可见K562细胞呈典型凋亡样改变．结论：Adi能明显抑制K562细胞增殖，并具有显著促进细胞凋亡的作用．
AIM： To observe the effect of Andi injection （Adi） on proliferation and apoptosis of erythroleukemia K562 cells. METHODS： The effect of Adi on proliferation and apoptosis of K562 cells was assayed by using MTT assay to measure the cell vitality （ A value ） , flow cytometer to detect cell apoptosis, electron microscopy to observe the morphological changes after the action of Adi. RESULTS： ① MTT method showed that within the range of （2.5 - 20.0） μg/mL, Adi obviously inhibited the proliferation of K562 cells in a dose-dependent manner, and 20.0μg/mL Adi which affected K562 cells for 36 h had the lowest A value; ② The apoptosis rates were 50% and 39% in the cells that had been treated with 10.0,20.0 μg/mL Adi, respectively. The apoptosis rates were significantly higher than that of control group （ 10%, P 〈 0. 05 ） ; ③ Typical apoptotic changes were found in K562 cells treated with Adi by electron microscope. CONCLUSION： Adi can inhibit the leukemic cell proliferation and induce the apoptosis of K562 cells.
出处
《第四军医大学学报》
北大核心
2007年第12期1081-1083,共3页
Journal of the Fourth Military Medical University
