摘要
目的:建立一种大鼠马尾神经急性受压性损伤的动物模型.方法:将36只SD大鼠随机分为对照组(A组),1d实验组(B组),7d实验组(C组)及21d实验组(D组),每组动物9只.将平头金属螺钉自L6椎板紧贴棘突拧入各实验组大鼠椎管,取术后1,7,21d不同时间的动物采用联合行为评分(CBS)、测定脊髓诱发电位(SCEP)潜伏期值进行综合评判,并取骶髓进行H-E染色,观察脊髓神经元形态学改变.结果:在螺钉拧入椎管后,H-E染色显示大鼠骶髓前角细胞发生明显的形态改变,术后的CBS评分1d:(17.89±1.27)分,7d:(9.78±1.30)分,21d:(9.33±1.41)分;脊髓诱发电位(SCEP)潜伏期值1d:(4.29±0.51)ms,7d:(4.35±0.54)ms,21d:(4.91±0.53)ms,与对照组相比较差异均有统计学意义(P<0.05).结论:建立的马尾急性压迫性损伤模型,操作简便,重复性好,为进一步研究马尾急性压迫损伤奠定了基础.
AIM： To establish an animal model of acute compressed cauda equina injury in the rat. METHODS： Thirty-six rats were randomly divided into control group （ Group A） , 1 d experimental group （Group B） , 7 d experimental group （Group C） and 21 d experimental group （Group D）,9 rats in each group. Metal screws without tip were used to drill into vertebral canal through the lamina of L6 sticking the spinous processes tightly in experimental groups. At different time （1,7,21 d） after operation, animal＇s comprehensive evaluation was carried out in combined behavioral score（CBS） , latency changes of spinal cord evoked potential （SCEP） and observation of the morphological changes in the sacral cords using HE staining under light microscopes. RESULTS ： HE staining showed the form of anterior horn cells changed significantly after the screw was drilled into the vertebral canal. At the different time points （1,7,21 d ） after operation, combined behavioral score （ 17.89 ± 1.27,9.78 ± 1.30,9.33 ±1.41） and SCEP latency （ms,4.29 ±0.51,4.35 ± 0.54, 4.91 ±0.53 ） were significantly different from those in the control group （ P 〈 0. 05 ）. CONCLUSION： Techniques for creating this acute compressed cauda equina injury model, are simple and can be highly replicable. It provides an available basis for the further study on acute compressed cauda equina injury.
出处
《第四军医大学学报》
北大核心
2007年第12期1078-1080,共3页
Journal of the Fourth Military Medical University
关键词
马尾
急性损伤
疾病模型
动物
大鼠
cauda equina
acute injury
disease models, animal
rats
