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玉簪新优品种离体培养技术的研究 被引量:8

Study on technique of in vitro culture of new and fine varieties of Hosta plantaginea
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摘要 以最新引进玉簪系列品种为试材进行离体快繁技术研究,结果表明:以休眠终期玉簪芽尖为外植体进行培养成功率为69.7%;适合多数品种的增殖培养基MS+6-BA 3.0 mg/L+IBA 0.1 mg/L,繁殖系数最高达4.9倍;适宜的生根培养基MS+ABT 0.3 mg/L,生根率达96.5%,同时生产周期缩短;草炭+珍珠岩(v/v=2/1)是玉簪生长较为理想的移栽基质。 The rapid propagation of newly introduced Hosta plantaginea varieties was studied by means of tissue culture. The results showed that when the shoot tips at the end of dormancy period were used as explants, the survival rate was 69.7%. The multiplication medium that was suitable for most varieties of Hosta plantaginea was MS + 3.0 mg/L 6-BA + 0.1 mg/L IBA, and the highest propagation coefficient reached 4.9 times. The appropriate rooting medium was MS + 0.3 mg/L ABT, and the rooting rate could be up to 96.5 %. In the meantime, the period of culture was shortened. Turf + perlite ( v/v = 2/1 ) was a good transplant substrate for the plantlet growth.
出处 《上海农业学报》 CSCD 2007年第2期77-80,共4页 Acta Agriculturae Shanghai
基金 2004年上海市绿化管理局项目(编号:F20040308-1)
关键词 玉簪 新优品种 快繁体系 Hosta plantaginea New and fine varieties Rapid propagation technology
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