摘要
分泌蛋白质组是指组织、细胞等分泌的全部蛋白质。分泌蛋白主要分布于体液和细胞质胞浆中,参与许多重要的生命过程。分泌蛋白质组的研究主要涉及分泌蛋白的制备、多维色谱或二维凝胶电泳分离、质谱鉴定及生物信息学分析等。本文主要介绍了分泌蛋白的合成途径、蛋白质组技术在分泌蛋白组研究中的应用、分泌蛋白组研究现状及存在的问题等。
Secretome is a almost kinds of body fluids group of all proteins secreted by cells and/or tissues. Secretory proteins exist in and extracellular fluid and participate in lots of important life processes. Secretomic research processes mainly involves in the preparation of secretory proteins, separation through multi-dimensional liquid chromatography or two-dimensional gel electrophoresis（2-DE）, identification by mass spectrometry and data mining using bioinformatic method. In this article the synthesis process of secretory protein is briefly summarized. The application of proteomic technologies and recent development in secretome were reviewed with emphasis on 2-DE and mass spectrometry-based methods and then the advance and perspectives of secretome were summarized.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第6期912-918,共7页
Chinese Journal of Analytical Chemistry
基金
北京市自然科学基金(No.7062042)
北京市重大项目(No.D0905001040231)资助项目
关键词
分泌蛋白
分泌蛋白质组
蛋白质组
质谱
二维凝胶电泳
Secretory protein, secretome, proteome, mass spectrometry, two-dimensional gel electrophoresis
