摘要
目的对流体剪切力作用下破骨细胞降钙素受体mRNA的表达水平进行研究。方法采用1α,25-(OH)2D3诱导5周龄SD大鼠骨髓细胞体外培养破骨细胞,观察不同流体剪切力对其形态的影响,运用荧光定量RT-PCR方法检测破骨细胞降钙素受体mRNA在不同力值下表达的差异。结果研究发现,破骨细胞在流体剪切力的作用下形态有较大改变,主要在细胞膜部位,可能与破骨细胞细胞骨架的适应性改变相关;流体剪切力对CTR mRNA的表达为上升调节(15mins组除外)。
Objective This paper focus on the influence of fluid shear stress on the osteoclast. Methods 1α,25 - （OH） 2 D3 was used to induce osteoclastogenesis from bone marrow cells and reliable cell culture methods was established. Osteoclasts after 6 days clutrue was loaded in different fluid shear stress and their morphilogical adaptation was observed. Fluorescent quantitative RT - PCR tool was used to detect the CTR expression level under different shear stress. Results and Conclusions： After loading, the osteoclasts showed their adaptive changes characterized of bulky body , transformation in membrane , particles mobilities inside and lucency changes. To CTR mRNA,the shear stress up -regulates its mRNA expression, the larger stress value was,the higher mRNA expression level.
出处
《安徽医学》
2007年第3期173-175,共3页
Anhui Medical Journal
基金
国家自然科学基金资助项目(30271428)
关键词
破骨细胞
流体剪切力
降钙素受体
Osteoclast
Fluid shear stress
Calcitonin receptor
