摘要
目的以人毛囊隆突细胞为种子细胞体外构建组织工程复合皮,在体观察其功能性修复全层皮肤缺损的可行性。方法胶原酶消化法体外分离培养人毛囊隆突细胞和毛乳头细胞,实验分为A、B两组。A组将毛囊隆突细胞与毛乳头细胞按1:2混合,接种于胶原包被的聚羟基乙酸纤维支架中;B组单纯接种相同数量的毛乳头细胞。而后覆盖角质形成细胞膜片,构成组织工程复合皮,移植于裸鼠全层皮肤缺损创面。观察创面愈合情况,分别于术后2、4、6周在光学显微镜下观察移植物组织学变化。结果组织工程复合皮能够有效修复A、B两组裸鼠全层皮肤缺损。术后2周,A、B组创面均可见完整的表皮及真皮结构。术后4～6周,A组复合皮表皮层明显增厚并形成基膜的钉突,可见毛囊样结构;B组仅表皮层有所增厚但基膜平整,未见钉突和毛囊结构形成。结论以聚羟基乙酸真皮基质为支架,用角质形成细胞、毛囊隆突细胞和毛乳头细胞共同构建的组织工程复合皮,可以有效修复裸鼠全层皮肤缺损。其中毛囊隆突细胞参与了创面解剖修复,同时可能引导组织结构和功能的修复。
To investigate the feasibility of fabricating tissue engineering skin with human hair follicle bulge cells （HFBCs） to repair full-thickness skin wound. Methods HFBCs and dermal papilla cells （DPCs） isolated from human fetal hair follicles by collagenase digestion were cultured, purified and passaged. PGA-collagen scaffolds as bioengineered dermis were randomly divided into A and B groups. The HFBCs and DPCs （1：2） were seeded in scaffolds of group A and the equal amount of DPCs was seeded in scaffolds of group B as control. Then the keratinocyte sheets were seeded onto the surfaces of the scaffolds as bioengineered epidermis, The tissue engineering skins were then transplanted to repair the full-thickness wound on the back of nude mice. The wound healing process was observed and the plant histological changes of the transplanted engineered skin was observed with light microscope on 2, 4, 6 post-operation weeks （POW）. Results The full-thickness defect of nude mice in A and B groups could be effectively repaired by bioengineered skins, On 2 POW, integral epidermal and dermal structures were observed in the wounds in A and B groups, with thin epithelial layer and basement membrane. On 4 POW, epithelial layer became thickening and fete pegs formation was observed in basement membrane in A group, but only thickening of epithelial layer was observed in B group. On 6 POW, rete pegs structure was seen to descend and hair-follicle-like structure was formed, while only thickened epithelial layer with flat basement membrane were formed in B group. Conclusion From the composite skin engineered with PGA-collagen hybrid scaffolds and keratnocytes ,HFBCs and DPCs could effectively repair the full-thickness skin defect of nude mice. The hair follicle stem cells participate in the process of anatomic repair of wound, and might be able to induce the repair of skin structure and function.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2007年第3期222-224,共3页
Chinese Journal of Burns
基金
全军医学科学技术研究"十一五"计划专项课题(06Z033)
关键词
干细胞
毛囊
组织工程
皮肤
人工
Stem cells
Hair follicle
Tissue engineering
Skin,artificial
