摘要
以分布于峨眉山不同山峪不同海拔（500-1800m）的17个鱼腥草（Houttuynia cordata Thunb.）居群及生长地土样作为试验材料，按土壤农化分析常规方法对土壤pH值及土壤营养元素进行测定，用水蒸馏法提取植株地上部分的挥发油，并用GCMS对挥发油化学成分进行分离和鉴定，峰面积归一法测定各成分含量。结果显示：峨眉山鱼腥草生长地土壤肥力水平较高，对鱼腥草的生长极为有利。从所有居群中共鉴定出35种化合物，各居群挥发油成分组成及含量存在较大的差异。月桂烯作为四个山峪唯一共有的主要成分在各居群中均能检测到，且在各居群中的平均含量最高。乙酸龙脑酯和trans-β-罗勒烯的含量与海拔高度间分别存在显著和极显著正相关。方差分析及多重比较的结果表明，不同山峪以及海拔高度对各居群挥发油成分和含量有显著影响。通过偏相关分析发现土壤微量元素对鱼腥草挥发油共有成分合成与积累的作用大于大量元素，微量元素中以有效钼的影响最为显著，大量元素中以速效钾的影响最为显著。采用逐步多元回归分析筛选出了对挥发油共有成分含量影响显著的土壤营养元素。
The aerial part of H. cordata and the soil of 17 populations were collected from different valleys and altitudes （500-- 1800m） of Mt. Emei. pH value and soil nutrient contents were determined by regular methods of agricultural chemistry analysis of the soil. Volatile oil was obtained by hydrodistillation from the aerial part of all materials. The chemical constituents were separated and identified by GC-MS, and the relative content of each constituent was determined by area normalization. The results showed that fertility of all the soil samples were higher, which was an advantage to the growth of H. cordata populaions. Totally, thirty-six components were identified. The components and content of volatile oil in different H. cordata populations were various. Myrcene, being the main component, could be detected in all populations, and its average content was higher than that of other chemical components. The content of bornyl acetate had positive correlation with the altitude and the content of trans-β-ocimene had significant positive correlation with the altitude. The results of variance analysis and multiple comparisons showed that the altitude had significant influence on the compositions and contents-of volatile oil of H. cordata populations. It was found that microelements have more important influence on volatile oil components than macroelements through partial correlation analysis. Available B is the most important influence factor in all microelements. Available K is the most important influence factor in all macroelements. Soil nutrients which have significant influence on 10 mutual volatile oil components were deduced by stepwise regression analysis.
出处
《生态学报》
CAS
CSCD
北大核心
2007年第6期2239-2250,共12页
Acta Ecologica Sinica
基金
四川省教育厅重点资助项目(2003A011)
教育部留学回国人员科研启动基金资助项目~~
关键词
鱼腥草
挥发油
居群
海拔
生态因子
土壤养分
气相色谱-质谱法
Houttuynia cordata Thunb.
volatile oil
population
altitude
ecology factors
soil nutrient
GC-MS
